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377d
From Proteopedia
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(New page: 200px<br /><applet load="377d" size="350" color="white" frame="true" align="right" spinBox="true" caption="377d, resolution 1.760Å" /> '''5'-R(*CP*GP*UP*AP*C...)
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Revision as of 19:37, 29 January 2008
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5'-R(*CP*GP*UP*AP*CP*G)-3'
Overview
The crystal structure of the alternating RNA r(CGUAC)dG with a 3'-terminal, deoxy G residue has been determined at 1.76 A resolution. The crystal, belongs to the orthorhombic space group C2221, unit-cell dimensions a =, 29.53, b = 44.61 and c = 94.18 A, with two independent duplexes (I and II), per asymmetric unit. The structure was solved by the molecular-replacement, method. The final R factor was 18.8% using 4757 reflections in the, resolution range 8.0-1.76 A. The model contains a total of 496 atoms and, 85 solvent molecules. The two duplexes form the repeating unit and stack, in the usual head-to-tail (5',3'/5',3') fashion into a pseudocontinuous, helical column. Almost all of the 2'-hydroxyl groups are engaged in the, three modes of water-mediated interactions to the base N3/O2 atoms, the, sugar O4' atoms and the backbone phosphates. Thus, the 2'-hydroxyl group, of RNA is probably contributing to the stability of the duplexes.
About this Structure
377D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
1.76 A structure of a pyrimidine start alternating A-RNA hexamer r(CGUAC)dG., Biswas R, Mitra SN, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):570-6. PMID:9761851
Page seeded by OCA on Tue Jan 29 21:37:11 2008
