1h8y
From Proteopedia
(New page: 200px<br /> <applet load="1h8y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h8y, resolution 2.0Å" /> '''CRYSTAL STRUCTURE OF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H8Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with SO4 and MER as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8Y OCA]]. | + | 1H8Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with SO4 and MER as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8Y OCA]]. |
==Reference== | ==Reference== | ||
Crystal structures of the class D beta-lactamase OXA-13 in the native form and in complex with meropenem., Pernot L, Frenois F, Rybkine T, L'Hermite G, Petrella S, Delettre J, Jarlier V, Collatz E, Sougakoff W, J Mol Biol. 2001 Jul 20;310(4):859-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11453693 11453693] | Crystal structures of the class D beta-lactamase OXA-13 in the native form and in complex with meropenem., Pernot L, Frenois F, Rybkine T, L'Hermite G, Petrella S, Delettre J, Jarlier V, Collatz E, Sougakoff W, J Mol Biol. 2001 Jul 20;310(4):859-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11453693 11453693] | ||
| + | [[Category: Beta-lactamase]] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxa-13]] | [[Category: oxa-13]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:22:56 2007'' |
Revision as of 10:18, 30 October 2007
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CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-13 IN COMPLEX WITH MEROPENEM
Overview
The therapeutic problems posed by class D beta-lactamases, a family of, serine enzymes that hydrolyse beta-lactam antibiotics following an, acylation-deacylation mechanism, are increased by the very low level of, sensitivity of these enzymes to beta-lactamase inhibitors. To gain, structural and mechanistic insights to aid the design of new inhibitors, we have determined the crystal structure of OXA-13 from Pseudomonas, aeruginosa in the apo form and in complex with the carbapenem meropenem., The native form consisted of a dimer displaying an overall organisation, similar to that found in the closely related enzyme OXA-10. In the, acyl-enzyme complex, the positioning of the antibiotic appeared to be, ensured mainly by (i) the covalent acyl bond and (ii) a strong salt-bridge, involving the ... [(full description)]
About this Structure
1H8Y is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with SO4 and MER as [ligands]. Active as [Beta-lactamase], with EC number [3.5.2.6]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [OCA].
Reference
Crystal structures of the class D beta-lactamase OXA-13 in the native form and in complex with meropenem., Pernot L, Frenois F, Rybkine T, L'Hermite G, Petrella S, Delettre J, Jarlier V, Collatz E, Sougakoff W, J Mol Biol. 2001 Jul 20;310(4):859-74. PMID:11453693
Page seeded by OCA on Tue Oct 30 12:22:56 2007
Categories: Beta-lactamase | Pseudomonas aeruginosa | Single protein | Collatz, E. | Delettre, J. | Frenois, F. | Hermite, G.L. | Jarlier, V. | Pernot, L. | Petrella, S. | Rybkine, T. | Sougakoff, W. | MER | SO4 | Acyl-enzyme | Class d | Hydrolase | Meropenem | Oxa-13
