409d
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(New page: 200px<br /><applet load="409d" size="350" color="white" frame="true" align="right" spinBox="true" caption="409d, resolution 2.50Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 19:42, 29 January 2008
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CRYSTAL STRUCTURE OF AN RNA R(CCCIUGGG) WITH THREE INDEPENDENT DUPLEXES INCORPORATING TANDEM I.U WOBBLES
Overview
The crystal structure of the RNA octamer duplex r(CCCIUGGG)2has been, elucidated at 2.5 A resolution. The crystals belong to the space group, P21and have unit cell constants a = 33.44 A, b = 43.41 A, c = 49.39 A and, beta = 104.7 degrees with three independent duplexes (duplexes 1-3) in the, asymmetric unit. The structure was solved by the molecular replacement, method and refined to an Rwork/Rfree of 0.185/0.243 using 3765 reflections, between 8.0 and 2.5 A. This is the first report of an RNA crystal, structure incorporating I.U wobbles and three molecules in the asymmetric, unit. Duplex 1 displays a kink of 24 degrees between the mismatch sites, while duplexes 2 and 3 have two kinks each of 19 degrees and 27 degrees, and 24 degrees and 29 degrees, respectively, on either side of the tandem, mismatches. At the I.U/U.I mismatch steps, duplex 1 has a twist angle of, 33.9 degrees, close to the average for all base pair steps, but duplexes 2, and 3 are underwound, with twist angles of 24.4 degrees and 26.5 degrees, respectively. The tandem I.U wobbles show intrastrand purine-pyrimidine, stacking but exhibit interstrand purine-purine stacking with the flanking, C.G pairs. The three independent duplexes are stacked non-coaxially in a, head-to-tail fashion to form infinite pseudo-continuous helical columns, which form intercolumn hydrogen bonding interactions through the, 2'-hydroxyl groups where the minor grooves come together.
About this Structure
409D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of an RNA octamer duplex r(CCCIUGGG)2 incorporating tandem I.U wobbles., Pan B, Mitra SN, Sun L, Hart D, Sundaralingam M, Nucleic Acids Res. 1998 Dec 15;26(24):5699-706. PMID:9838002
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