417d
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(New page: 200px<br /><applet load="417d" size="350" color="white" frame="true" align="right" spinBox="true" caption="417d, resolution 1.500Å" /> '''A THYMINE-LIKE BASE...)
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Revision as of 19:42, 29 January 2008
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A THYMINE-LIKE BASE ANALOGUE FORMS WOBBLE PAIRS WITH ADENINE
Overview
The DNA hexamer d(CACGPG), in which dP is the ambivalent pyrimidine, nucleoside analogue 2'-deoxy-beta-d-ribofuranosyl-(6H,8H-3, 4-dihydropyrimido[4,5-c][1,2]oxazin-7-one), crystallises as a left-handed, Z-DNA duplex. X-ray analysis at 1.5 A shows that both P. A base-pairs are, of the wobble type. This result appears inconsistent with other evidence, from hybridisation and NMR studies of P-containing oligonucleotides, which, suggests that, while P can form stable base-pairs with either A or G, thymine-like properties are more pronounced. Thermal denaturation, experiments over a range of solution pH values indicate that protonation, of the P.A base-pairs is unlikely to be responsible for the anomalous, behaviour. No specific crystal packing effects can be identfied as an, explanation, and it is concluded that base stacking and other interactions, between nucleotide residues in Z-DNA are responsible.
About this Structure
417D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A thymine-like base analogue forms wobble pairs with adenine in a Z-DNA duplex., Schuerman GS, Van Meervelt L, Loakes D, Brown DM, Kong Thoo Lin P, Moore MH, Salisbury SA, J Mol Biol. 1998 Oct 9;282(5):1005-11. PMID:9753550
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