422d

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Revision as of 19:42, 29 January 2008

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spinqualitylabelsall models 422d, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'

Overview

The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at, 2.6 A resolution by the molecular-replacement method and refined to an, R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer, crystallized in the monoclinic space group C2, with unit-cell parameters a, = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent, strands in the asymmetric unit. The dodecamer adopts an octamer duplex, structure with four 5'-overhang residues (G1A2U3C4), which form, Watson-Crick base pairs with another four 5'-overhang residues of a, symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its, center four mismatched base pairs flanked by two Watson-Crick base pairs., The mismatched bases form two G.U wobble base pairs at the ends and two, U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U), and a water bridge connecting the N(3) of the cytosine and uridine. The, present study reinforces the concept of the stability of the conformation, of UUCG in RNA double-helical structures.

About this Structure

422D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

Reference

Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides., Eswaramoorthy S, Rao ST, Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):8-12. Epub 2003 Dec, 18. PMID:14684886

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