437d

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Revision as of 19:43, 29 January 2008

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spinqualitylabelsall models 437d, resolution 1.6Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF AN RNA PSEUDOKNOT FROM BEET WESTERN YELLOW VIRUS INVOLVED IN RIBOSOMAL FRAMESHIFTING

Overview

Many viruses regulate translation of polycistronic mRNA using a -1, ribosomal frameshift induced by an RNA pseudoknot. A pseudoknot has two, stems that form a quasi-continuous helix and two connecting loops. A 1.6 A, crystal structure of the beet western yellow virus (BWYV) pseudoknot, reveals rotation and a bend at the junction of the two stems. A loop base, is inserted in the major groove of one stem with quadruple-base, interactions. The second loop forms a new minor-groove triplex motif with, the other stem, involving 2'-OH and triple-base interactions, as well as, sodium ion coordination. Overall, the number of hydrogen bonds stabilizing, the tertiary interactions exceeds the number involved in Watson-Crick base, pairs. This structure will aid mechanistic analyses of ribosomal, frameshifting.

About this Structure

437D is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.

Reference

Minor groove RNA triplex in the crystal structure of a ribosomal frameshifting viral pseudoknot., Su L, Chen L, Egli M, Berger JM, Rich A, Nat Struct Biol. 1999 Mar;6(3):285-92. PMID:10074948

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