469d
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(New page: 200px<br /><applet load="469d" size="350" color="white" frame="true" align="right" spinBox="true" caption="469d, resolution 1.7Å" /> '''CRYSTAL STRUCTURE AND...)
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Revision as of 19:44, 29 January 2008
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CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA
Overview
2'-O-(2-Methoxyethyl)-RNA (MOE-RNA) is a nucleic acid analog with, promising features for antisense applications. Compared with, phosphorothioate DNA (PS-DNA), the MOE modification offers improved, nuclease resistance, enhanced RNA affinity, improved cellular uptake and, intestinal absorption, reduced toxicity and immune stimulation. The, crystal structure of a fully modified MOE-RNA dodecamer duplex, (CGCGAAUUCGCG) was determined at 1.7 A resolution. In the majority of the, MOE substituents, the torsion angle around the ethylene alkyl chain, assumes a gauche conformation. The conformational preorganization of the, MOE groups is consistent with the improved RNA affinity and the extensive, hydration of the substituents could play a role in the improved cellular, uptake of MOE-RNA. A specific hydration pattern that bridges substituent, and phosphate oxygen atoms in the minor groove of MOE-RNA may explain its, high nuclease resistance.
About this Structure
469D is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA., Teplova M, Minasov G, Tereshko V, Inamati GB, Cook PD, Manoharan M, Egli M, Nat Struct Biol. 1999 Jun;6(6):535-9. PMID:10360355
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