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1h8v
From Proteopedia
(New page: 200px<br /> <applet load="1h8v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h8v, resolution 1.90Å" /> '''THE X-RAY CRYSTAL S...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H8V is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]] with NAG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8V OCA]]. | + | 1H8V is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Hypocrea_jecorina Hypocrea jecorina]] with NAG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Cellulase Cellulase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4]]. Structure known Active Sites: CA1, CA2, CA3, CA4, CA5 and CA6. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8V OCA]]. |
==Reference== | ==Reference== | ||
The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution., Sandgren M, Shaw A, Ropp TH, Wu S, Bott R, Cameron AD, Stahlberg J, Mitchinson C, Jones TA, J Mol Biol. 2001 Apr 27;308(2):295-310. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11327768 11327768] | The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution., Sandgren M, Shaw A, Ropp TH, Wu S, Bott R, Cameron AD, Stahlberg J, Mitchinson C, Jones TA, J Mol Biol. 2001 Apr 27;308(2):295-310. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11327768 11327768] | ||
| + | [[Category: Cellulase]] | ||
[[Category: Hypocrea jecorina]] | [[Category: Hypocrea jecorina]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: trichoderma reesei cel12a]] | [[Category: trichoderma reesei cel12a]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:23:44 2007'' |
Revision as of 10:19, 30 October 2007
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THE X-RAY CRYSTAL STRUCTURE OF THE TRICHODERMA REESEI FAMILY 12 ENDOGLUCANASE 3, CEL12A, AT 1.9 A RESOLUTION
Overview
We present the three-dimensional structure of Trichoderma reesei, endoglucanase 3 (Cel12A), a small, 218 amino acid residue (24.5 kDa), neutral pI, glycoside hydrolase family 12 cellulase that lacks a, cellulose-binding module. The structure has been determined using X-ray, crystallography and refined to 1.9 A resolution. The asymmetric unit, consists of six non-crystallographic symmetry-related molecules that were, exploited to improve initial multiple isomorphous replacement phasing, and, subsequent structure refinement. The enzyme contains one disulfide bridge, and is glycosylated at Asp164 by a single N-acetyl glucosamine residue., The protein has the expected fold for a glycoside hydrolase clan-C family, 12 enzyme. It contains two beta-sheets, of six and nine strands, packed on, top ... [(full description)]
About this Structure
1H8V is a [Single protein] structure of sequence from [Hypocrea jecorina] with NAG as [ligand]. Active as [Cellulase], with EC number [3.2.1.4]. Structure known Active Sites: CA1, CA2, CA3, CA4, CA5 and CA6. Full crystallographic information is available from [OCA].
Reference
The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution., Sandgren M, Shaw A, Ropp TH, Wu S, Bott R, Cameron AD, Stahlberg J, Mitchinson C, Jones TA, J Mol Biol. 2001 Apr 27;308(2):295-310. PMID:11327768
Page seeded by OCA on Tue Oct 30 12:23:44 2007
Categories: Cellulase | Hypocrea jecorina | Single protein | Bott, R. | Cameron, A.D. | Jones, T.A. | Mitchinson, C. | Ropp, T.H. | Sandgren, M. | Shaw, A. | Stahlberg, J. | Wu, S. | NAG | Cellulose degradation | Endoglucanase | Gh family 12 | Glycosyl hydrolase | Hydrolase | Trichoderma reesei cel12a
