5bna

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Revision as of 19:47, 29 January 2008

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spinqualitylabelsall models 5bna, resolution 2.600Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

THE PRIMARY MODE OF BINDING OF CISPLATIN TO A B-DNA DODECAMER: C-G-C-G-A-A-T-T-C-G-C-G

Overview

When cisplatin [cis- diamminodichloroplatinum (II)] is diffused into, pre-grown crystals of the B-DNA double-helical dodecamer, C-G-C-G-A-A-T-T-C-G-C-G, it binds preferentially to the N7 positions of, guanines, with what probably is an aquo bridge between Pt and the adjacent, O6 atom of the same guanine. The entire guanine ring moves slightly toward, the platinum site, into the major groove. Only three of the eight, potential cisplatin binding sites on guanines actually are occupied, and, this differential reactivity can be explained in terms of the relative, freedom of motion of guanines toward the major groove. This shift of, guanines upon ligation may weaken the glycosyl bond and assist in the, depurination that leads to mismatch SOS repair and G.C. to T.A., transversion.

About this Structure

5BNA is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

The primary mode of binding of cisplatin to a B-DNA dodecamer: C-G-C-G-A-A-T-T-C-G-C-G., Wing RM, Pjura P, Drew HR, Dickerson RE, EMBO J. 1984 May;3(5):1201-6. PMID:6539674

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