This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
8psh
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="8psh" size="350" color="white" frame="true" align="right" spinBox="true" caption="8psh" /> '''HIGH RESOLUTION NMR STRUCTURE OF THE STEREOR...)
Next diff →
Revision as of 19:53, 29 January 2008
|
HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE
Overview
In this work, we present the first NMR solution structure of a DNA/RNA, hybrid containing stereoregular Rp-phosphorothioate modifications of all, DNA backbone linkages. The complex of the enzymatically synthesized, phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary, RNA r(CCUGACGC) was found to adopt an overall conformation within the, A-form family. Most helical parameters and the sugar puckers of the DNA, strand assume values intermediate between A- and B-form. The close, structural similarity with the unmodified DNA/RNA hybrid of the same, sequence may explain why both the natural and the sulfur-substituted, complex can be recognized and digested by ribonuclease H.
About this Structure
8PSH is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a stereoregular phosphorothioate DNA/RNA duplex., Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H, Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216
Page seeded by OCA on Tue Jan 29 21:53:16 2008
