8psh
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(New page: 200px<br /><applet load="8psh" size="350" color="white" frame="true" align="right" spinBox="true" caption="8psh" /> '''HIGH RESOLUTION NMR STRUCTURE OF THE STEREOR...)
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Revision as of 19:53, 29 January 2008
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HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE
Overview
In this work, we present the first NMR solution structure of a DNA/RNA, hybrid containing stereoregular Rp-phosphorothioate modifications of all, DNA backbone linkages. The complex of the enzymatically synthesized, phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary, RNA r(CCUGACGC) was found to adopt an overall conformation within the, A-form family. Most helical parameters and the sugar puckers of the DNA, strand assume values intermediate between A- and B-form. The close, structural similarity with the unmodified DNA/RNA hybrid of the same, sequence may explain why both the natural and the sulfur-substituted, complex can be recognized and digested by ribonuclease H.
About this Structure
8PSH is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of a stereoregular phosphorothioate DNA/RNA duplex., Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H, Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216
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