2iwn

From Proteopedia

Revision as of 08:57, 31 January 2008

3RD PDZ DOMAIN OF MULTIPLE PDZ DOMAIN PROTEIN MPDZ (CASP TARGET)

Overview

PDZ domains are protein-protein interaction modules that generally bind to, the C termini of their target proteins. The C-terminal four amino acids of, a prospective binding partner of a PDZ domain are typically the, determinants of binding specificity. In an effort to determine the, structures of a number of PDZ domains we have included appropriate four, residue extensions on the C termini of PDZ domain truncation mutants, designed for self-binding. Multiple truncations of each PDZ domain were, generated. The four residue extensions, which represent known specificity, sequences of the target PDZ domains and cover both class I and II motifs, form intermolecular contacts in the expected manner for the interactions, of PDZ domains with protein C termini for both classes. We present the, structures of eight unique PDZ domains crystallized using this approach, and focus on four which provide information on selectivity (PICK1 and the, third PDZ domain of DLG2), binding site flexibility (the third PDZ domain, of MPDZ), and peptide-domain interactions (MPDZ 12th PDZ domain). Analysis, of our results shows a clear improvement in the chances of obtaining PDZ, domain crystals by using this approach compared to similar truncations of, the PDZ domains without the C-terminal four residue extensions.

About this Structure

2IWN is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of PICK1 and other PDZ domains obtained with the help of self-binding C-terminal extensions., Elkins JM, Papagrigoriou E, Berridge G, Yang X, Phillips C, Gileadi C, Savitsky P, Doyle DA, Protein Sci. 2007 Apr;16(4):683-94. PMID:17384233

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