1as8

From Proteopedia

---

Revision as of 13:50, 29 October 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 1as8, resolution 1.85Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE OF NITRITE BOUND TO REDUCED ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE

Overview

The structures of oxidized, reduced, nitrite-soaked oxidized and, nitrite-soaked reduced nitrite reductase from Alcaligenes faecalis have, been determined at 1.8-2.0 A resolution using data collected at -160, degrees C. The active site at cryogenic temperature, as at room, temperature, contains a tetrahedral type II copper site liganded by three, histidines and a water molecule. The solvent site is empty when crystals, are reduced with ascorbate. A fully occupied oxygen-coordinate nitrite, occupies the solvent site in crystals soaked in nitrite. Ascorbate-reduced, crystals soaked in a glycerol-methanol solution and nitrite at -40 degrees, C remain colorless at -160 degrees C but turn amber-brown when warmed, suggesting that NO is released. Nitrite is found at one-half occupancy., Five new ... [(full description)]

About this Structure

1AS8 is a [Single protein] structure of sequence from [Alcaligenes faecalis] with CU and NO2 as [ligands]. Active as [[1]], with EC number [1.7.99.3]. Full crystallographic information is available from [OCA].

Reference

Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. Mechanistic implications., Murphy ME, Turley S, Adman ET, J Biol Chem. 1997 Nov 7;272(45):28455-60. PMID:9353305

Page seeded by OCA on Mon Oct 29 15:54:53 2007