2qm8
From Proteopedia
(New page: 200px<br /><applet load="2qm8" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qm8, resolution 1.70Å" /> '''MeaB, A Bacterial Ho...) |
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caption="2qm8, resolution 1.70Å" /> | caption="2qm8, resolution 1.70Å" /> | ||
'''MeaB, A Bacterial Homolog of MMAA, in the Nucleotide Free Form'''<br /> | '''MeaB, A Bacterial Homolog of MMAA, in the Nucleotide Free Form'''<br /> | ||
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| + | ==Overview== | ||
| + | MeaB is an auxiliary protein that plays a crucial role in the protection, and assembly of the B(12)-dependent enzyme methylmalonyl-CoA mutase., Impairments in the human homologue of MeaB, MMAA, lead to methylmalonic, aciduria, an inborn error of metabolism. To explore the role of this, metallochaperone, its structure was solved in the nucleotide-free form, as, well as in the presence of product, GDP. MeaB is a homodimer, with each, subunit containing a central alpha/beta-core G domain that is typical of, the GTPase family, as well as alpha-helical extensions at the N and C, termini that are not found in other metalloenzyme chaperone GTPases. The, C-terminal extension appears to be essential for nucleotide-independent, dimerization, and the N-terminal region is implicated in protein-protein, interaction with its partner protein, methylmalonyl-CoA mutase. The, structure of MeaB confirms that it is a member of the G3E family of P-loop, GTPases, which contains other putative metallochaperones HypB, CooC, and, UreG. Interestingly, the so-called switch regions, responsible for signal, transduction following GTP hydrolysis, are found at the dimer interface of, MeaB instead of being positioned at the surface of the protein where its, partner protein methylmalonyl-CoA mutase should bind. This observation, suggests a large conformation change of MeaB must occur between the GDP-, and GTP-bound forms of this protein. Because of their high sequence, homology, the missense mutations in MMAA that result in methylmalonic, aciduria have been mapped onto MeaB and, in conjunction with mutagenesis, data, provide possible explanations for the pathology of this disease. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2QM8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QM8 OCA]. | + | 2QM8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Po4 Binding Site For Residue B 1500'>AC1</scene>, <scene name='pdbsite=AC2:Po4 Binding Site For Residue A 1501'>AC2</scene>, <scene name='pdbsite=AC3:Po4 Binding Site For Residue A 1502'>AC3</scene> and <scene name='pdbsite=AC4:Po4 Binding Site For Residue A 1503'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QM8 OCA]. |
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| + | ==Reference== | ||
| + | Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria., Hubbard PA, Padovani D, Labunska T, Mahlstedt SA, Banerjee R, Drennan CL, J Biol Chem. 2007 Oct 26;282(43):31308-16. Epub 2007 Aug 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17728257 17728257] | ||
[[Category: Methylobacterium extorquens]] | [[Category: Methylobacterium extorquens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: metallochaperone]] | [[Category: metallochaperone]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 10:59:05 2008'' |
Revision as of 08:59, 31 January 2008
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MeaB, A Bacterial Homolog of MMAA, in the Nucleotide Free Form
Overview
MeaB is an auxiliary protein that plays a crucial role in the protection, and assembly of the B(12)-dependent enzyme methylmalonyl-CoA mutase., Impairments in the human homologue of MeaB, MMAA, lead to methylmalonic, aciduria, an inborn error of metabolism. To explore the role of this, metallochaperone, its structure was solved in the nucleotide-free form, as, well as in the presence of product, GDP. MeaB is a homodimer, with each, subunit containing a central alpha/beta-core G domain that is typical of, the GTPase family, as well as alpha-helical extensions at the N and C, termini that are not found in other metalloenzyme chaperone GTPases. The, C-terminal extension appears to be essential for nucleotide-independent, dimerization, and the N-terminal region is implicated in protein-protein, interaction with its partner protein, methylmalonyl-CoA mutase. The, structure of MeaB confirms that it is a member of the G3E family of P-loop, GTPases, which contains other putative metallochaperones HypB, CooC, and, UreG. Interestingly, the so-called switch regions, responsible for signal, transduction following GTP hydrolysis, are found at the dimer interface of, MeaB instead of being positioned at the surface of the protein where its, partner protein methylmalonyl-CoA mutase should bind. This observation, suggests a large conformation change of MeaB must occur between the GDP-, and GTP-bound forms of this protein. Because of their high sequence, homology, the missense mutations in MMAA that result in methylmalonic, aciduria have been mapped onto MeaB and, in conjunction with mutagenesis, data, provide possible explanations for the pathology of this disease.
About this Structure
2QM8 is a Single protein structure of sequence from Methylobacterium extorquens with as ligand. Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria., Hubbard PA, Padovani D, Labunska T, Mahlstedt SA, Banerjee R, Drennan CL, J Biol Chem. 2007 Oct 26;282(43):31308-16. Epub 2007 Aug 28. PMID:17728257
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