3bh0
From Proteopedia
(New page: 200px<br /><applet load="3bh0" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bh0, resolution 2.35Å" /> '''ATPase Domain of G40...) |
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caption="3bh0, resolution 2.35Å" /> | caption="3bh0, resolution 2.35Å" /> | ||
'''ATPase Domain of G40P'''<br /> | '''ATPase Domain of G40P'''<br /> | ||
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| + | ==Overview== | ||
| + | Helicases are essential enzymes for DNA replication, a fundamental process, in all living organisms. The DnaB family are hexameric replicative, helicases that unwind duplex DNA and coordinate with RNA primase and other, proteins at the replication fork in prokaryotes. Here, we report the, full-length crystal structure of G40P, a DnaB family helicase. The hexamer, complex reveals an unusual architectural feature and a new type of, assembly mechanism. The hexamer has two tiers: a three-fold symmetric, N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with, two different conformations, termed cis and trans, come together to, provide a topological solution for the dual symmetry within a hexamer., Structure-guided mutational studies indicate an important role for the, N-terminal tier in binding primase and regulating primase-mediated, stimulation of helicase activity. This study provides insights into the, structural and functional interplay between G40P helicase and DnaG, primase. | ||
==About this Structure== | ==About this Structure== | ||
| - | 3BH0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 3BH0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_sp6 Enterobacteria phage sp6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BH0 OCA]. |
| - | [[Category: | + | |
| + | ==Reference== | ||
| + | The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18157148 18157148] | ||
| + | [[Category: Enterobacteria phage sp6]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chen, X.S.]] | [[Category: Chen, X.S.]] | ||
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[[Category: replication]] | [[Category: replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 11:02:34 2008'' |
Revision as of 09:02, 31 January 2008
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ATPase Domain of G40P
Overview
Helicases are essential enzymes for DNA replication, a fundamental process, in all living organisms. The DnaB family are hexameric replicative, helicases that unwind duplex DNA and coordinate with RNA primase and other, proteins at the replication fork in prokaryotes. Here, we report the, full-length crystal structure of G40P, a DnaB family helicase. The hexamer, complex reveals an unusual architectural feature and a new type of, assembly mechanism. The hexamer has two tiers: a three-fold symmetric, N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with, two different conformations, termed cis and trans, come together to, provide a topological solution for the dual symmetry within a hexamer., Structure-guided mutational studies indicate an important role for the, N-terminal tier in binding primase and regulating primase-mediated, stimulation of helicase activity. This study provides insights into the, structural and functional interplay between G40P helicase and DnaG, primase.
About this Structure
3BH0 is a Single protein structure of sequence from Enterobacteria phage sp6. Full crystallographic information is available from OCA.
Reference
The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:18157148
Page seeded by OCA on Thu Jan 31 11:02:34 2008
