1a8s
From Proteopedia
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| - | [[Image:1a8s.gif|left|200px]]<br /><applet load="1a8s" size=" | + | [[Image:1a8s.gif|left|200px]]<br /><applet load="1a8s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1a8s, resolution 1.80Å" /> | caption="1a8s, resolution 1.80Å" /> | ||
'''CHLOROPEROXIDASE F/PROPIONATE COMPLEX'''<br /> | '''CHLOROPEROXIDASE F/PROPIONATE COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1A8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens] with SO4 and PPI as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=NUL:Catalytic Triad'>NUL</scene>. Full crystallographic information is available from [http:// | + | 1A8S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=PPI:'>PPI</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] Known structural/functional Site: <scene name='pdbsite=NUL:Catalytic+Triad'>NUL</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A8S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: propionate complex]] | [[Category: propionate complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:29:35 2008'' |
Revision as of 07:29, 3 February 2008
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CHLOROPEROXIDASE F/PROPIONATE COMPLEX
Overview
The structures of cofactor-free haloperoxidases from Streptomyces, aureofaciens, Streptomyces lividans, and Pseudomonas fluorescens have been, determined at resolutions between 1.9 A and 1.5 A. The structures of two, enzymes complexed with benzoate or propionate identify the binding site, for the organic acids which are required for the haloperoxidase activity., Based on these complexes and on the structure of an inactive variant, a, reaction mechanism is proposed for the halogenation reaction with, peroxoacid and hypohalous acid as reaction intermediates. Comparison of, the structures suggests that a specific halide binding site is absent in, the enzymes but that hydrophobic organic compounds may fit into the active, site pocket for halogenation at preferential sites.
About this Structure
1A8S is a Single protein structure of sequence from Pseudomonas fluorescens with and as ligands. Active as Chloride peroxidase, with EC number 1.11.1.10 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural investigation of the cofactor-free chloroperoxidases., Hofmann B, Tolzer S, Pelletier I, Altenbuchner J, van Pee KH, Hecht HJ, J Mol Biol. 1998 Jun 19;279(4):889-900. PMID:9642069
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