1joa
From Proteopedia
(New page: 200px<br /> <applet load="1joa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1joa, resolution 2.8Å" /> '''NADH PEROXIDASE WITH...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1JOA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]] with FAD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.1 1.11.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JOA OCA]]. | + | 1JOA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]] with FAD as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/NADH_peroxidase NADH peroxidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.1 1.11.1.1]]. Structure known Active Site: ACT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JOA OCA]]. |
==Reference== | ==Reference== | ||
Structure of the native cysteine-sulfenic acid redox center of enterococcal NADH peroxidase refined at 2.8 A resolution., Yeh JI, Claiborne A, Hol WG, Biochemistry. 1996 Aug 6;35(31):9951-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8756456 8756456] | Structure of the native cysteine-sulfenic acid redox center of enterococcal NADH peroxidase refined at 2.8 A resolution., Yeh JI, Claiborne A, Hol WG, Biochemistry. 1996 Aug 6;35(31):9951-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8756456 8756456] | ||
[[Category: Enterococcus faecalis]] | [[Category: Enterococcus faecalis]] | ||
| + | [[Category: NADH peroxidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Claiborne, A.C.]] | [[Category: Claiborne, A.C.]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:27:13 2007'' |
Revision as of 10:22, 30 October 2007
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NADH PEROXIDASE WITH CYSTEINE-SULFENIC ACID
Overview
In order to obtain the crystal structure of the flavoprotein NADH, peroxidase with its native Cys42-sulfenic acid redox center, a strategy, combining reduced exposure of crystals to ambient oxygen and data, collection at -160 degrees C was applied. The structure of the native, enzyme to 2.8 A resolution is described; these results conclusively, establish the existence of the Cys42-sulfenic acid as the functional, non-flavin redox center of the peroxidase and provide the first structure, for any naturally occurring protein-sulfenic acid. The Cys42-sulfenic acid, atoms C alpha-C beta-S gamma-O roughly define a planar arrangement which, is stacked parallel to the si face of the FAD isoalloxazine and positions, the sulfenyl oxygen atom only 3.3 A from FAD-C4A. His10-N epsilon 2, contributes a ... [(full description)]
About this Structure
1JOA is a [Single protein] structure of sequence from [Enterococcus faecalis] with FAD as [ligand]. Active as [NADH peroxidase], with EC number [1.11.1.1]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].
Reference
Structure of the native cysteine-sulfenic acid redox center of enterococcal NADH peroxidase refined at 2.8 A resolution., Yeh JI, Claiborne A, Hol WG, Biochemistry. 1996 Aug 6;35(31):9951-7. PMID:8756456
Page seeded by OCA on Tue Oct 30 12:27:13 2007
