1cow
From Proteopedia
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| - | [[Image:1cow.gif|left|200px]]<br /><applet load="1cow" size=" | + | [[Image:1cow.gif|left|200px]]<br /><applet load="1cow" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1cow, resolution 3.1Å" /> | caption="1cow, resolution 3.1Å" /> | ||
'''BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH AUROVERTIN B'''<br /> | '''BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH AUROVERTIN B'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1COW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with MG, ANP, ADP and AUR as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34] Known structural/functional Sites: <scene name='pdbsite=CAT:The Carboxylate Group Of GLU Residue Is Believed To Acti ...'>CAT</scene> and <scene name='pdbsite=PLP:The Residue Listed Is The LYS Within The P-Loop (Phospha ...'>PLP</scene>. Full crystallographic information is available from [http:// | + | 1COW is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=ANP:'>ANP</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=AUR:'>AUR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34] Known structural/functional Sites: <scene name='pdbsite=CAT:The+Carboxylate+Group+Of+GLU+Residue+Is+Believed+To+Acti+...'>CAT</scene> and <scene name='pdbsite=PLP:The+Residue+Listed+Is+The+LYS+Within+The+P-Loop+(Phospha+...'>PLP</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1COW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrogen ion transport]] | [[Category: hydrogen ion transport]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:34:47 2008'' |
Revision as of 07:34, 3 February 2008
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BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH AUROVERTIN B
Overview
In the structure of bovine mitochondrial F1-ATPase that was previously, determined with crystals grown in the presence of, adenylyl-imidodiphosphate (AMP-PNP) and ADP, the three catalytic, beta-subunits have different conformations and nucleotide occupancies., Adenylyl-imidodiphosphate is bound to one beta-subunit (betaTP), ADP is, bound to the second (betaDP), and no nucleotide is bound to the third, (betaE). Here we show that the uncompetitive inhibitor aurovertin B binds, to bovine F1 at two equivalent sites in betaTP and betaE, in a cleft, between the nucleotide binding and C-terminal domains. In betaDP, the, aurovertin B pocket is incomplete and is inaccessible to the inhibitor., The aurovertin B bound to betaTP interacts with alpha-Glu399 in the, adjacent alphaTP subunit, whereas the aurovertin B bound to betaE is too, distant from alphaE to make an equivalent interaction. Both sites, encompass betaArg-412, which was shown by mutational studies to be, involved in binding aurovertin. Except for minor changes around the, aurovertin pockets, the structure of bovine F1-ATPase is the same as, determined previously. Aurovertin B appears to act by preventing closure, of the catalytic interfaces, which is essential for a catalytic mechanism, involving cyclic interconversion of catalytic sites.
About this Structure
1COW is a Protein complex structure of sequences from Bos taurus with , , and as ligands. Active as Transferred entry: 3.6.3.14, with EC number 3.6.1.34 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
The structure of bovine F1-ATPase complexed with the antibiotic inhibitor aurovertin B., van Raaij MJ, Abrahams JP, Leslie AG, Walker JE, Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):6913-7. PMID:8692918
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