1dpo
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1dpo.gif|left|200px]]<br /><applet load="1dpo" size=" | + | [[Image:1dpo.gif|left|200px]]<br /><applet load="1dpo" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dpo, resolution 1.59Å" /> | caption="1dpo, resolution 1.59Å" /> | ||
'''STRUCTURE OF RAT TRYPSIN'''<br /> | '''STRUCTURE OF RAT TRYPSIN'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1DPO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with CA, SO4 and BEN as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Known structural/functional Site: <scene name='pdbsite=CAT:Catalytic Triad'>CAT</scene>. Full crystallographic information is available from [http:// | + | 1DPO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_rattus Rattus rattus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BEN:'>BEN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Known structural/functional Site: <scene name='pdbsite=CAT:Catalytic+Triad'>CAT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DPO OCA]. |
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: zymogen]] | [[Category: zymogen]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:35:36 2008'' |
Revision as of 07:35, 3 February 2008
|
STRUCTURE OF RAT TRYPSIN
Overview
The structure of a rat trypsin mutant [S195C] at a temperature of 120 K, has been refined to a crystallographic R factor of 17.4% between 12.0 and, 1.59 A and is compared with the structure of the D102N mutant at 295 K. A, reduction in the unit cell dimensions in going from room temperature to, low temperature is accompanied by a decrease in molecular surface area and, radius of gyration. The overall structure remains similar to that at room, temperature. The attainable resolution appears to be improved due to the, decrease in the fall off of intensities with resolution [reduction of the, temperature factor]. This decreases the uncertainty in the atomic, positions and allows the localization of more protein atoms and solvent, molecules in the low temperature map. The largest differences between the, two models occur at residues with higher than average temperature factors., Several features can be localized in the solvent region of the 120 K map, that are not seen in the 295 K map. These include several more water, molecules as well as an interstitial sulfate ion and two interstitial, benzamidine molecules.
About this Structure
1DPO is a Single protein structure of sequence from Rattus rattus with , and as ligands. Active as Trypsin, with EC number 3.4.21.4 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
1.59 A structure of trypsin at 120 K: comparison of low temperature and room temperature structures., Earnest T, Fauman E, Craik CS, Stroud R, Proteins. 1991;10(3):171-87. PMID:1881877
Page seeded by OCA on Sun Feb 3 09:35:36 2008
Categories: Rattus rattus | Single protein | Trypsin | Stroud, R.M. | BEN | CA | SO4 | Digestion | Hydrolase | Multigene family | Pancreas | Serine protease | Signal | Zymogen
