1dwq
From Proteopedia
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| - | [[Image:1dwq.jpg|left|200px]]<br /><applet load="1dwq" size=" | + | [[Image:1dwq.jpg|left|200px]]<br /><applet load="1dwq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1dwq, resolution 2.2Å" /> | caption="1dwq, resolution 2.2Å" /> | ||
'''CRYSTAL STRUCTURE OF HYDROXYNITRILE LYASE FROM MANIHOT ESCULENTA IN COMPLEX WITH SUBSTRATES ACETONE AND CHLOROACETONE:IMPLICATIONS FOR THE MECHANISM OF CYANOGENESIS'''<br /> | '''CRYSTAL STRUCTURE OF HYDROXYNITRILE LYASE FROM MANIHOT ESCULENTA IN COMPLEX WITH SUBSTRATES ACETONE AND CHLOROACETONE:IMPLICATIONS FOR THE MECHANISM OF CYANOGENESIS'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DWQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Manihot_esculenta Manihot esculenta] with ATO as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.3.2.4 Transferred entry: 3.3.2.4], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.37 4.2.1.37] Known structural/functional Sites: <scene name='pdbsite=ASA:Catalytic Triad Active Site'>ASA</scene> and <scene name='pdbsite=ASB:Catalytic Triad Active Site'>ASB</scene>. Full crystallographic information is available from [http:// | + | 1DWQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Manihot_esculenta Manihot esculenta] with <scene name='pdbligand=ATO:'>ATO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_3.3.2.4 Transferred entry: 3.3.2.4], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.37 4.2.1.37] Known structural/functional Sites: <scene name='pdbsite=ASA:Catalytic+Triad+Active+Site'>ASA</scene> and <scene name='pdbsite=ASB:Catalytic+Triad+Active+Site'>ASB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DWQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydroxynitrile lyase]] | [[Category: hydroxynitrile lyase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:35:52 2008'' |
Revision as of 07:35, 3 February 2008
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CRYSTAL STRUCTURE OF HYDROXYNITRILE LYASE FROM MANIHOT ESCULENTA IN COMPLEX WITH SUBSTRATES ACETONE AND CHLOROACETONE:IMPLICATIONS FOR THE MECHANISM OF CYANOGENESIS
Overview
The crystal structures of hydroxynitrile lyase from Manihot esculenta, (MeHNL) complexed with the native substrate acetone and substrate analogue, chloroacetone have been determined and refined at 2.2 A resolution. The, substrates are positioned in the active site by hydrogen-bond interactions, of the carbonyl O atom with Thr11 OG, Ser80 OG and, to a lesser extent, Cys81 SG. These studies support a mechanism for cyanogenesis as well as, for the stereospecific MeHNL-catalyzed formation of (S)-cyanohydrins, which closely resembles the base-catalyzed chemical reaction of HCN with, carbonyl compounds.
About this Structure
1DWQ is a Single protein structure of sequence from Manihot esculenta with as ligand. Active as Transferred entry: 3.3.2.4, with EC number 4.2.1.37 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Structure of hydroxynitrile lyase from Manihot esculenta in complex with substrates acetone and chloroacetone: implications for the mechanism of cyanogenesis., Lauble H, Forster S, Miehlich B, Wajant H, Effenberger F, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):194-200. PMID:11173464
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