1e0x
From Proteopedia
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| - | [[Image:1e0x.jpg|left|200px]]<br /><applet load="1e0x" size=" | + | [[Image:1e0x.jpg|left|200px]]<br /><applet load="1e0x" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1e0x, resolution 1.65Å" /> | caption="1e0x, resolution 1.65Å" /> | ||
'''XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A'''<br /> | '''XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1E0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans] with GOL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Known structural/functional Sites: <scene name='pdbsite=AC1:X2f Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Xys Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:X2f Binding Site For Chain B'>AC3</scene>, <scene name='pdbsite=AC4:Xys Binding Site For Chain B'>AC4</scene>, <scene name='pdbsite=ACA:Catalytic Acid/Base Chain A'>ACA</scene>, <scene name='pdbsite=ACB:Catalytic Acid/Base Chain B'>ACB</scene>, <scene name='pdbsite=NUA:Catalytic Nucleophile Chain A'>NUA</scene> and <scene name='pdbsite=NUB:Catalytic Nucleophile Chain B'>NUB</scene>. Full crystallographic information is available from [http:// | + | 1E0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Known structural/functional Sites: <scene name='pdbsite=AC1:X2f+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Xys+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:X2f+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:Xys+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=ACA:Catalytic+Acid/Base+Chain+A'>ACA</scene>, <scene name='pdbsite=ACB:Catalytic+Acid/Base+Chain+B'>ACB</scene>, <scene name='pdbsite=NUA:Catalytic+Nucleophile+Chain+A'>NUA</scene> and <scene name='pdbsite=NUB:Catalytic+Nucleophile+Chain+B'>NUB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E0X OCA]. |
==Reference== | ==Reference== | ||
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[[Category: xylanase]] | [[Category: xylanase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:36:29 2008'' |
Revision as of 07:36, 3 February 2008
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XYLANASE 10A FROM SREPTOMYCES LIVIDANS. XYLOBIOSYL-ENZYME INTERMEDIATE AT 1.65 A
Overview
Endoxylanases are a group of enzymes that hydrolyze the beta-1, 4-linked, xylose backbone of xylans. They are predominantly found in two discrete, sequence families known as glycoside hydrolase families 10 and 11. The, Streptomyces lividans xylanase Xyl10A is a family 10 enzyme, the native, structure of which has previously been determined by x-ray crystallography, at a 2.6 A resolution (Derewenda, U., Swenson, L., Green, R., Wei, Y., Morosoli, R., Shareck, F., Kluepfel, D., and Derewenda, Z. S. (1994) J., Biol. Chem. 269, 20811-20814). Here, we report the native structure of, Xyl10A refined at a resolution of 1.2 A, which reveals many features such, as the rare occurrence of a discretely disordered disulfide bond between, residues Cys-168 and Cys-201. In order to investigate substrate binding, and specificity in glycoside hydrolase family 10, the covalent xylobiosyl, enzyme and the covalent cellobiosyl enzyme intermediates of Xyl10A were, trapped through the use of appropriate 2-fluoroglycosides. The, alpha-linked intermediate with the nucleophile, Glu-236, is in a (4)C(1), chair conformation as previously observed in the family 10 enzyme Cex from, Cellulomonas fimi (Notenboom, V., Birsan, C., Warren, R. A. J., Withers, S. G., and Rose, D. R. (1998) Biochemistry 37, 4751-4758). The different, interactions of Xyl10A with the xylobiosyl and cellobiosyl moieties, notably conformational changes in the -2 and -1 subsites, together with, the observed kinetics on a range of aryl glycosides, shed new light on, substrate specificity in glycoside hydrolase family 10.
About this Structure
1E0X is a Single protein structure of sequence from Streptomyces lividans with as ligand. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Known structural/functional Sites: , , , , , , and . Full crystallographic information is available from OCA.
Reference
Substrate specificity in glycoside hydrolase family 10. Structural and kinetic analysis of the Streptomyces lividans xylanase 10A., Ducros V, Charnock SJ, Derewenda U, Derewenda ZS, Dauter Z, Dupont C, Shareck F, Morosoli R, Kluepfel D, Davies GJ, J Biol Chem. 2000 Jul 28;275(30):23020-6. PMID:10930426
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Categories: Endo-1,4-beta-xylanase | Single protein | Streptomyces lividans | Charnock, S.J. | Dauter, Z. | Davies, G.J. | Derewenda, U. | Derewenda, Z.S. | Ducros, V. | Dupont, C. | Kluepfel, D. | Morosoli, R. | Shareck, F. | GOL | Glycoside hydrolase family 10 | Glycosyl-enzyme intermediate | Xylan degradation | Xylanase
