1e30
From Proteopedia
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| - | [[Image:1e30.gif|left|200px]]<br /><applet load="1e30" size=" | + | [[Image:1e30.gif|left|200px]]<br /><applet load="1e30" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1e30, resolution 1.50Å" /> | caption="1e30, resolution 1.50Å" /> | ||
'''CRYSTAL STRUCTURE OF THE MET148GLN MUTANT OF RUSTICYANIN AT 1.5 ANGSTROM RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF THE MET148GLN MUTANT OF RUSTICYANIN AT 1.5 ANGSTROM RESOLUTION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1E30 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=CUA:Cu Site Chain A'>CUA</scene> and <scene name='pdbsite=CUB:Cu Site Chain B'>CUB</scene>. Full crystallographic information is available from [http:// | + | 1E30 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=CUA:Cu+Site+Chain+A'>CUA</scene> and <scene name='pdbsite=CUB:Cu+Site+Chain+B'>CUB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E30 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rusticyanin]] | [[Category: rusticyanin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:36:47 2008'' |
Revision as of 07:36, 3 February 2008
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CRYSTAL STRUCTURE OF THE MET148GLN MUTANT OF RUSTICYANIN AT 1.5 ANGSTROM RESOLUTION
Overview
The small blue copper protein rusticyanin from Thiobacillus ferrooxidans, contains a type 1 Cu centre with a single axial ligand, Met148, which, together with the His-Cys-His trigonal planar ligands produces a distorted, trigonal pyramidal coordination geometry to copper. Type 1 Cu sites are, found in cupredoxins and several multicopper proteins, including oxidases, and nitrite reductases. The role of the axial ligand has been extensively, debated in terms of its function in the fine tuning of the redox potential, and spectroscopic properties of type 1 Cu sites. Numerous mutations of the, Met ligand in azurins have been studied, but interpretation of the results, has been complicated by the presence of the additional carbonyl oxygen, ligand from Gly45, a neighbouring residue to the coordinating His46. The, importance of the axial ligand has been further emphasized by the finding, that the type 1 centre in Rhus vernicifera stellacyanin, with the lowest, redox potential in a type 1 Cu site of 184 mV, has Gln as the axial, ligand, whilst fungal laccase and ceruloplasmin, which have redox, potentials of 550-800 mV, have a Leu in this position. Here, the crystal, structure of the M148Q mutant of rusticyanin at 1.5 A resolution is, presented. This is a significantly higher resolution than that of the, structures of native rusticyanin. In addition, the M148Q structure is that, of the oxidized protein while the native structures to date are of the, reduced protein. The mutant protein crystallizes with two molecules per, asymmetric unit, in contrast to the one present in the native crystal, form. This mutant's redox potential (550 mV at pH 3.2) is lowered compared, with that of the native protein ( approximately 670 mV at pH 3.2) by about, 120 mV. The type 1 Cu site of M148Q closely mimics the structural, characteristics of the equivalent site in non-glycosylated cucumber, stellacyanin (redox potential approximately 260 mV) and, owing to the, absence in rusticyanin of the fifth, carbonyl ligand present in azurin, may provide a better model for the R. vernicifera stellacyanin (redox, potential approximately 184 mV) type 1 Cu site, which also lacks the fifth, ligand. Furthermore, the presence of two molecules in the asymmetric unit, cell indicates a potential binding region of the redox partners.
About this Structure
1E30 is a Single protein structure of sequence from Acidithiobacillus ferrooxidans with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Structure of the M148Q mutant of rusticyanin at 1.5 A: a model for the copper site of stellacyanin., Hough MA, Hall JF, Kanbi LD, Hasnain SS, Acta Crystallogr D Biol Crystallogr. 2001 Mar;57(Pt 3):355-60. PMID:11223511
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