1ecb
From Proteopedia
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| - | [[Image:1ecb.jpg|left|200px]]<br /><applet load="1ecb" size=" | + | [[Image:1ecb.jpg|left|200px]]<br /><applet load="1ecb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ecb, resolution 2.7Å" /> | caption="1ecb, resolution 2.7Å" /> | ||
'''ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 GMP, 1 MG PER SUBUNIT'''<br /> | '''ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 GMP, 1 MG PER SUBUNIT'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ECB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and 5GP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amidophosphoribosyltransferase Amidophosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.14 2.4.2.14] Known structural/functional Sites: <scene name='pdbsite=NTA:Ntn Amidotransferase Active Site'>NTA</scene>, <scene name='pdbsite=NTB:Ntn Amidotransferase Active Site'>NTB</scene>, <scene name='pdbsite=NTC:Ntn Amidotransferase Active Site'>NTC</scene>, <scene name='pdbsite=NTD:Ntn Amidotransferase Active Site'>NTD</scene>, <scene name='pdbsite=PRA:Phosphoribosyl transf. Active Site. The Active Site In T ...'>PRA</scene>, <scene name='pdbsite=PRB:Phosphoribosyl transf. Active Site. The Active Site In T ...'>PRB</scene>, <scene name='pdbsite=PRC:Phosphoribosyl transf. Active Site. The Active Site In T ...'>PRC</scene> and <scene name='pdbsite=PRT:Phosphoribosyl transf. Active Site. The Active Site In T ...'>PRT</scene>. Full crystallographic information is available from [http:// | + | 1ECB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=5GP:'>5GP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Amidophosphoribosyltransferase Amidophosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.14 2.4.2.14] Known structural/functional Sites: <scene name='pdbsite=NTA:Ntn+Amidotransferase+Active+Site'>NTA</scene>, <scene name='pdbsite=NTB:Ntn+Amidotransferase+Active+Site'>NTB</scene>, <scene name='pdbsite=NTC:Ntn+Amidotransferase+Active+Site'>NTC</scene>, <scene name='pdbsite=NTD:Ntn+Amidotransferase+Active+Site'>NTD</scene>, <scene name='pdbsite=PRA:Phosphoribosyl+transf.+Active+Site.+The+Active+Site+In+T+...'>PRA</scene>, <scene name='pdbsite=PRB:Phosphoribosyl+transf.+Active+Site.+The+Active+Site+In+T+...'>PRB</scene>, <scene name='pdbsite=PRC:Phosphoribosyl+transf.+Active+Site.+The+Active+Site+In+T+...'>PRC</scene> and <scene name='pdbsite=PRT:Phosphoribosyl+transf.+Active+Site.+The+Active+Site+In+T+...'>PRT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:39:02 2008'' |
Revision as of 07:39, 3 February 2008
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ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 GMP, 1 MG PER SUBUNIT
Overview
Activation of gluatmine phosphoribosylpyrophosphate (RPPP), amidotransferase (GPATase) by binding of a PRPP substrate analog results, in the formation of a 20 A channel connecting the active site for, glutamine hydrolysis in one domain with the PRPP site in a second domain., This solvent-inaccessible channel permits transfer of the NH3 intermediate, between the two active sites. Tunneling of NH3 may be a common mechanism, for glutamine amidotransferase-catalyzed nitrogen transfer and for, coordination of catalysis at two distinct active sites in complex enzymes., The 2.4 A crystal structure of the active conformer of GPATase also, provides the first description of an intact active site for the, phosphoribosyltransferase (PRTase) family of nucleotide synthesis and, salvage enzymes. Chemical assistance to catalysis is provided primarily by, the substrate and secondarily by the enzyme in the proposed, structure-based mechanism. Different catalytic and inhibitory modes of, divalent cation binding to the PRTase active site are revealed in the, active conformer of the enzyme and in a feedback-inhibited GMP complex.
About this Structure
1ECB is a Single protein structure of sequence from Escherichia coli with and as ligands. Active as Amidophosphoribosyltransferase, with EC number 2.4.2.14 Known structural/functional Sites: , , , , , , and . Full crystallographic information is available from OCA.
Reference
Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site., Krahn JM, Kim JH, Burns MR, Parry RJ, Zalkin H, Smith JL, Biochemistry. 1997 Sep 16;36(37):11061-8. PMID:9333323
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