1ecj
From Proteopedia
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| - | [[Image:1ecj.jpg|left|200px]]<br /><applet load="1ecj" size=" | + | [[Image:1ecj.jpg|left|200px]]<br /><applet load="1ecj" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ecj, resolution 2.5Å" /> | caption="1ecj, resolution 2.5Å" /> | ||
'''ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 AMP PER TETRAMER'''<br /> | '''ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 AMP PER TETRAMER'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ECJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with AMP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Amidophosphoribosyltransferase Amidophosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.14 2.4.2.14] Known structural/functional Sites: <scene name='pdbsite=NTA:Ntn Amidotransferase Active Site'>NTA</scene>, <scene name='pdbsite=NTB:Ntn Amidotransferase Active Site'>NTB</scene>, <scene name='pdbsite=NTC:Ntn Amidotransferase Active Site'>NTC</scene>, <scene name='pdbsite=NTD:Ntn Amidotransferase Active Site'>NTD</scene>, <scene name='pdbsite=PRA:Phosphoribosyl transf. Active Site. The Active Site In T ...'>PRA</scene>, <scene name='pdbsite=PRB:Phosphoribosyl transf. Active Site. The Active Site In T ...'>PRB</scene>, <scene name='pdbsite=PRC:Phosphoribosyl transf. Active Site. The Active Site In T ...'>PRC</scene> and <scene name='pdbsite=PRT:Phosphoribosyl transf. Active Site. The Active Site In T ...'>PRT</scene>. Full crystallographic information is available from [http:// | + | 1ECJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AMP:'>AMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Amidophosphoribosyltransferase Amidophosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.14 2.4.2.14] Known structural/functional Sites: <scene name='pdbsite=NTA:Ntn+Amidotransferase+Active+Site'>NTA</scene>, <scene name='pdbsite=NTB:Ntn+Amidotransferase+Active+Site'>NTB</scene>, <scene name='pdbsite=NTC:Ntn+Amidotransferase+Active+Site'>NTC</scene>, <scene name='pdbsite=NTD:Ntn+Amidotransferase+Active+Site'>NTD</scene>, <scene name='pdbsite=PRA:Phosphoribosyl+transf.+Active+Site.+The+Active+Site+In+T+...'>PRA</scene>, <scene name='pdbsite=PRB:Phosphoribosyl+transf.+Active+Site.+The+Active+Site+In+T+...'>PRB</scene>, <scene name='pdbsite=PRC:Phosphoribosyl+transf.+Active+Site.+The+Active+Site+In+T+...'>PRC</scene> and <scene name='pdbsite=PRT:Phosphoribosyl+transf.+Active+Site.+The+Active+Site+In+T+...'>PRT</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:39:04 2008'' |
Revision as of 07:39, 3 February 2008
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ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE COMPLEXED WITH 2 AMP PER TETRAMER
Overview
Crystal structures of glutamine phosphoribosylpyrophosphate (PRPP), amidotransferase from Escherichia coli have been determined to 2.0-A, resolution in the absence of ligands, and to 2.5-A resolution with the, feedback inhibitor AMP bound to the PRPP catalytic site. Glutamine PRPP, amidotransferase (GPATase) employs separate catalytic domains to abstract, nitrogen from the amide of glutamine and to transfer nitrogen to the, acceptor substrate PRPP. The unliganded and AMP-bound structures, which, are essentially identical, are interpreted as the inhibited form of the, enzyme because the two active sites are disconnected and the PRPP active, site is solvent exposed. The structures were compared with a previously, reported 3.0-A structure of the homologous Bacillus subtilis enzyme (Smith, JL et al., 1994, Science 264:1427-1433). The comparison indicates a, pattern of conservation of peptide structures involved with catalysis and, variability in enzyme regulatory functions. Control of glutaminase, activity, communication between the active sites, and regulation by, feedback inhibitors are addressed differently by E. coli and B. subtilis, GPATases. The E. coli enzyme is a prototype for the metal-free GPATases, whereas the B. subtilis enzyme represents the metal-containing enzymes., The structure of the E. coli enzyme suggests that a common ancestor of the, two enzyme subfamilies may have included an Fe-S cluster.
About this Structure
1ECJ is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Amidophosphoribosyltransferase, with EC number 2.4.2.14 Known structural/functional Sites: , , , , , , and . Full crystallographic information is available from OCA.
Reference
Crystal structure of glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli., Muchmore CR, Krahn JM, Kim JH, Zalkin H, Smith JL, Protein Sci. 1998 Jan;7(1):39-51. PMID:9514258
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