1h3g
From Proteopedia
(New page: 200px<br /> <applet load="1h3g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h3g, resolution 2.1Å" /> '''CYCLOMALTODEXTRINASE...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1H3G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Flavobacterium_sp._92 Flavobacterium sp. 92]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.54 3.2.1.54]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H3G OCA]]. | + | 1H3G is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Flavobacterium_sp._92 Flavobacterium sp. 92]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Cyclomaltodextrinase Cyclomaltodextrinase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.54 3.2.1.54]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H3G OCA]]. |
==Reference== | ==Reference== | ||
Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92., Fritzsche HB, Schwede T, Schulz GE, Eur J Biochem. 2003 May;270(10):2332-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12752453 12752453] | Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92., Fritzsche HB, Schwede T, Schulz GE, Eur J Biochem. 2003 May;270(10):2332-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12752453 12752453] | ||
| + | [[Category: Cyclomaltodextrinase]] | ||
[[Category: Flavobacterium sp. 92]] | [[Category: Flavobacterium sp. 92]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:29:38 2007'' |
Revision as of 10:24, 30 October 2007
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CYCLOMALTODEXTRINASE FROM FLAVOBACTERIUM SP. NO. 92: FROM DNA SEQUENCE TO PROTEIN STRUCTURE
Overview
Starting with oligopeptide sequences and using PCR, the gene of the, cyclodextrinase from Flavobacterium sp. no. 92 was derived from the, genomic DNA. The gene was sequenced and expressed in Escherichia coli; the, gene product was purified and crystallized. An X-ray diffraction analysis, using seleno-methionines with multiwavelength anomalous diffraction, techniques yielded the refined 3D structure at 2.1 A resolution. The, enzyme hydrolyzes alpha(1,4)-glycosidic bonds of cyclodextrins and linear, malto-oligosaccharides. It belongs to the glycosylhydrolase family no. 13, and has a chain fold similar to that of alpha-amylases, cyclodextrin, glycosyltransferases, and other cyclodextrinases. In contrast with most, family members but in agreement with other cyclodextrinases, the enzyme, ... [(full description)]
About this Structure
1H3G is a [Single protein] structure of sequence from [Flavobacterium sp. 92] with CA as [ligand]. Active as [Cyclomaltodextrinase], with EC number [3.2.1.54]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92., Fritzsche HB, Schwede T, Schulz GE, Eur J Biochem. 2003 May;270(10):2332-41. PMID:12752453
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