1gjq
From Proteopedia
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| - | [[Image:1gjq.jpg|left|200px]]<br /><applet load="1gjq" size=" | + | [[Image:1gjq.jpg|left|200px]]<br /><applet load="1gjq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gjq, resolution 2.7Å" /> | caption="1gjq, resolution 2.7Å" /> | ||
'''PSEUDOMONAS AERUGINOSA CD1 NITRITE REDUCTASE REDUCED CYANIDE COMPLEX'''<br /> | '''PSEUDOMONAS AERUGINOSA CD1 NITRITE REDUCTASE REDUCED CYANIDE COMPLEX'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CYN, HEC and DHE as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.2 1.9.3.2] Known structural/functional Site: <scene name='pdbsite=HEA:Cn Binding Site For Chain B'>HEA</scene>. Full crystallographic information is available from [http:// | + | 1GJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CYN:'>CYN</scene>, <scene name='pdbligand=HEC:'>HEC</scene> and <scene name='pdbligand=DHE:'>DHE</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Transferred_entry:_1.7.2.1 Transferred entry: 1.7.2.1], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.2 1.9.3.2] Known structural/functional Site: <scene name='pdbsite=HEA:Cn+Binding+Site+For+Chain+B'>HEA</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: reductase]] | [[Category: reductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:40:28 2008'' |
Revision as of 07:40, 3 February 2008
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PSEUDOMONAS AERUGINOSA CD1 NITRITE REDUCTASE REDUCED CYANIDE COMPLEX
Overview
Cyanide binding to fully reduced Pseudomonas aeruginosa cd(1) nitrite, reductase (Pa cd(1) NiR) has been investigated for the wild-type enzyme, and a site-directed mutant in which the active-site His369 was replaced by, Ala. This mutation reduces the affinity toward cyanide (by approximately, 13-fold) and especially decreases the rate of binding of cyanide to the, reduced d(1) heme (by approximately 100-fold). The crystal structure of, wild-type reduced Pa cd(1) NiR saturated with cyanide was determined to a, resolution of 2.7 A. Cyanide binds to the iron of the d(1) heme, with an, Fe-C-N angle of 168 degrees for both subunits of the dimer and only His369, is within hydrogen bonding distance of the nitrogen atom of the ligand., These results suggest that in Pa cd(1) NiR the invariant distal residue, His369 plays a dominant role in controlling the binding of anionic ligands, and allow the discussion of the mechanism of cyanide binding to the, wild-type enzyme.
About this Structure
1GJQ is a Single protein structure of sequence from Pseudomonas aeruginosa with , and as ligands. Active as Transferred entry: 1.7.2.1, with EC number 1.9.3.2 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Cyanide binding to cd(1) nitrite reductase from Pseudomonas aeruginosa: role of the active-site His369 in ligand stabilization., Sun W, Arese M, Brunori M, Nurizzo D, Brown K, Cambillau C, Tegoni M, Cutruzzola F, Biochem Biophys Res Commun. 2002 Feb 15;291(1):1-7. PMID:11829453
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