1gjr
From Proteopedia
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| - | [[Image:1gjr.jpg|left|200px]]<br /><applet load="1gjr" size=" | + | [[Image:1gjr.jpg|left|200px]]<br /><applet load="1gjr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gjr, resolution 2.1Å" /> | caption="1gjr, resolution 2.1Å" /> | ||
'''FERREDOXIN-NADP+ REDUCTASE COMPLEXED WITH NADP+ BY COCRYSTALLIZATION'''<br /> | '''FERREDOXIN-NADP+ REDUCTASE COMPLEXED WITH NADP+ BY COCRYSTALLIZATION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GJR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.] with FAD and NAP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Known structural/functional Site: <scene name='pdbsite=FAD:Nap Binding Site For Chain A'>FAD</scene>. Full crystallographic information is available from [http:// | + | 1GJR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Anabaena_sp. Anabaena sp.] with <scene name='pdbligand=FAD:'>FAD</scene> and <scene name='pdbligand=NAP:'>NAP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ferredoxin--NADP(+)_reductase Ferredoxin--NADP(+) reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.18.1.2 1.18.1.2] Known structural/functional Site: <scene name='pdbsite=FAD:Nap+Binding+Site+For+Chain+A'>FAD</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:40:29 2008'' |
Revision as of 07:40, 3 February 2008
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FERREDOXIN-NADP+ REDUCTASE COMPLEXED WITH NADP+ BY COCRYSTALLIZATION
Overview
The flavoenzyme ferredoxin-NADP+ reductase (FNR) catalyses the production, of NADPH in photosynthesis. The three-dimensional structure of FNR, presents two distinct domains, one for binding of the FAD prosthetic group, and the other for NADP+ binding. In spite of extensive experiments and, different crystallographic approaches, many aspects about how the NADP+, substrate binds to FNR and how the hydride ion is transferred from FAD to, NADP+ remain unclear. The structure of an FNR:NADP+ complex from Anabaena, has been determined by X-ray diffraction analysis of the cocrystallised, units to 2.1 A resolution. Structural perturbation of FNR induced by, complex formation produces a narrower cavity in which the 2'-phospho-AMP, and pyrophosphate portions of the NADP+ are perfectly bound. In addition, the nicotinamide mononucleotide moiety is placed in a new pocket created, near the FAD cofactor with the ribose being in a tight conformation. The, crystal structure of this FNR:NADP+ complex obtained by cocrystallisation, displays NADP+ in an unusual conformation and can be considered as an, intermediate state in the process of coenzyme recognition and binding., Structural analysis and comparison with previously reported complexes, allow us to postulate a mechanism which would permit efficient hydride, transfer to occur. Besides, this structure gives new insights into the, postulated formation of the ferredoxin:FNR:NADP+ ternary complex by, prediction of new intermolecular interactions, which could only exist, after FNR:NADP+ complex formation. Finally, structural comparison with the, members of the broad FNR structural family also provides an explanation, for the high specificity exhibited by FNR for NADP+/H versus NAD+/H.
About this Structure
1GJR is a Single protein structure of sequence from Anabaena sp. with and as ligands. Active as Ferredoxin--NADP(+) reductase, with EC number 1.18.1.2 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Mechanism of coenzyme recognition and binding revealed by crystal structure analysis of ferredoxin-NADP+ reductase complexed with NADP+., Hermoso JA, Mayoral T, Faro M, Gomez-Moreno C, Sanz-Aparicio J, Medina M, J Mol Biol. 2002 Jun 21;319(5):1133-42. PMID:12079352
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Categories: Anabaena sp. | Ferredoxin--NADP(+) reductase | Single protein | Gomez-Moreno, C. | Hermoso, J.A. | Mayoral, T. | Medina, M. | Sanz-Aparicio, J. | FAD | NAP | Fad | Flavoprotein | Fnr | Nadp | Nadp reductase | Oxidoreductase
