1gjy
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1gjy.gif|left|200px]]<br /><applet load="1gjy" size=" | + | [[Image:1gjy.gif|left|200px]]<br /><applet load="1gjy" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gjy, resolution 2.2Å" /> | caption="1gjy, resolution 2.2Å" /> | ||
'''THE X-RAY STRUCTURE OF THE SORCIN CALCIUM BINDING DOMAIN (SCBD) PROVIDES INSIGHT INTO THE PHOSPHORYLATION AND CALCIUM DEPENDENT PROCESSESS'''<br /> | '''THE X-RAY STRUCTURE OF THE SORCIN CALCIUM BINDING DOMAIN (SCBD) PROVIDES INSIGHT INTO THE PHOSPHORYLATION AND CALCIUM DEPENDENT PROCESSESS'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1GJY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_longicaudatus Cricetulus longicaudatus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain C The Sulfate Binding Site Is ...'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1GJY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_longicaudatus Cricetulus longicaudatus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+C+The+Sulfate+Binding+Site+Is+...'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GJY OCA]. |
==Reference== | ==Reference== | ||
| Line 22: | Line 22: | ||
[[Category: phosphorylation]] | [[Category: phosphorylation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:40:33 2008'' |
Revision as of 07:40, 3 February 2008
|
THE X-RAY STRUCTURE OF THE SORCIN CALCIUM BINDING DOMAIN (SCBD) PROVIDES INSIGHT INTO THE PHOSPHORYLATION AND CALCIUM DEPENDENT PROCESSESS
Overview
Sorcin is a 21.6 kDa calcium binding protein, expressed in a number of, mammalian tissues that belongs to the small, recently identified, penta-EF-hand (PEF) family. Like all members of this family, sorcin, undergoes a Ca2+-dependent translocation from cytosol to membranes where, it binds to target proteins. For sorcin, the targets differ in different, tissues, indicating that it takes part in a number of Ca2+-regulated, processes. The sorcin monomer is organized in two domains like in all PEF, proteins: a flexible, hydrophobic, glycine-rich N-terminal region and a, calcium binding C-terminal domain. In vitro, the PEF proteins are dimeric, in their Ca2+-free form, but have a marked tendency to precipitate when, bound to calcium. Stabilization of the dimeric structure is achieved by, pairing of the uneven EF-hand, EF5. Sorcin can also form tetramers at acid, pH.The sorcin calcium binding domain (SCBD, residues 33-198) expressed in, Escherichia coli was crystallized in the Ca2+-free form. The structure was, solved by molecular replacement and was refined to 2.2 A with a, crystallographic R-factor of 22.4 %. Interestingly, the asymmetric unit, contains two dimers.The structure of the SCBD leads to a model that, explains the solution properties and describes the Ca2+-induced, conformational changes. Phosphorylation studies show that the N-terminal, domain hinders phosphorylation of SCBD, i.e. the rate of phosphorylation, increased twofold in the absence of the N-terminal region. In addition, previous fluorescence studies indicated that hydrophobic residues are, exposed to solvent upon Ca2+ binding to full-length sorcin. The model, accounts for these data by proposing that Ca2+ binding weakens the, interactions between the two domains and leads to their reorientation, which exposes hydrophobic regions facilitating the Ca2+-dependent binding, to target proteins at or near membranes.
About this Structure
1GJY is a Single protein structure of sequence from Cricetulus longicaudatus with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The crystal structure of the sorcin calcium binding domain provides a model of Ca2+-dependent processes in the full-length protein., Ilari A, Johnson KA, Nastopoulos V, Verzili D, Zamparelli C, Colotti G, Tsernoglou D, Chiancone E, J Mol Biol. 2002 Mar 29;317(3):447-58. PMID:11922676
Page seeded by OCA on Sun Feb 3 09:40:33 2008
