1gm6
From Proteopedia
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| - | [[Image:1gm6.gif|left|200px]]<br /><applet load="1gm6" size=" | + | [[Image:1gm6.gif|left|200px]]<br /><applet load="1gm6" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gm6, resolution 2.13Å" /> | caption="1gm6, resolution 2.13Å" /> | ||
'''3-D STRUCTURE OF A SALIVARY LIPOCALIN FROM BOAR'''<br /> | '''3-D STRUCTURE OF A SALIVARY LIPOCALIN FROM BOAR'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GM6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with NAG, CD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=CAV:Nag Binding Site For Chain A'>CAV</scene>. Full crystallographic information is available from [http:// | + | 1GM6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=CAV:Nag+Binding+Site+For+Chain+A'>CAV</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GM6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: salivary lipocalin]] | [[Category: salivary lipocalin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:40:56 2008'' |
Revision as of 07:40, 3 February 2008
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3-D STRUCTURE OF A SALIVARY LIPOCALIN FROM BOAR
Overview
The X-ray structure of variant A of authentic boar salivary lipocalin, (SAL), a pheromone-binding protein specifically expressed in the, submaxillary glands of the boar, has been solved and refined at 2.1 A, resolution. The structure displays a classical lipocalin fold with a, nine-stranded sandwiched beta barrel and an alpha helix. A putative, glycosylation site, at position 53, has been found to carry a GlcNAc sugar, residue. In contrast with what was expected on the basis of mass, spectroscopy reports, the internal cavity was found to be devoid of bound, pheromonal compound (androstenone or androstenol). Instead, a small, electron density volume could be satisfied by a glycerol molecule, a, component of the cryoprotecting liquor. The internal cavity was revealed, to be very small for steroid compound accommodation. Therefore, docking, and molecular dynamics experiments were performed with both pheromonal, compounds. These simulations clearly demonstrate a volume increase of the, cavity upon steroid binding and the adaptation of the amino-acid side, chains to the steroid molecules. This explains the higher affinity of SAL, for both steroid molecules compared to other smaller molecules, although, no specific interaction is established with either compound.
About this Structure
1GM6 is a Single protein structure of sequence from Sus scrofa with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Boar salivary lipocalin. Three-dimensional X-ray structure and androsterol/androstenone docking simulations., Spinelli S, Vincent F, Pelosi P, Tegoni M, Cambillau C, Eur J Biochem. 2002 May;269(10):2449-56. PMID:12027882
Page seeded by OCA on Sun Feb 3 09:40:56 2008
Categories: Single protein | Sus scrofa | Cambillau, C. | Pelosi, P. | Spinelli, S. | Tegoni, M. | Vincent, F. | CD | GOL | NAG | Odorant binding protein | Salivary lipocalin
