1go7
From Proteopedia
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| - | [[Image:1go7.jpg|left|200px]]<br /><applet load="1go7" size=" | + | [[Image:1go7.jpg|left|200px]]<br /><applet load="1go7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1go7, resolution 2.10Å" /> | caption="1go7, resolution 2.10Å" /> | ||
'''THE METZINCIN'S METHIONINE: PRTC M226C-E189K DOUBLE MUTANT'''<br /> | '''THE METZINCIN'S METHIONINE: PRTC M226C-E189K DOUBLE MUTANT'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GO7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with CA, ZN and PO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Po4 Binding Site For Chain P'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1GO7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Po4+Binding+Site+For+Chain+P'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GO7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protease]] | [[Category: protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:41:21 2008'' |
Revision as of 07:41, 3 February 2008
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THE METZINCIN'S METHIONINE: PRTC M226C-E189K DOUBLE MUTANT
Overview
Serralysins are a family of metalloproteases secreted by Gram-negative, bacteria into the medium in the form of inactive zymogens. Usually, all, serralysin secretors have on the same operon a gene coding for a, periplasmic 10-kDa protein, which is an inhibitor of the secreted, protease. The recent characterization of the inhibitor of the alkaline, protease from Pseudomonas aeruginosa revealed a surprisingly low, dissociation constant of 4 pm, contrary to earlier studies on homologous, systems, where inhibition constants in the microm range were reported. To, approach a more accurate understanding, the crystal structure of the, complex between inhibitor and protease from P. aeruginosa was determined, at 1.74 A resolution and refined to R(free) = 0.204. The structure, reported here shows clearly that the N terminus of the inhibitor forms a, coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc, distance of 2.17 A. We conclude that this interaction adds substantially, to the complex stability and show also that similar interactions are found, in other metzincin-inhibitor complexes.
About this Structure
1GO7 is a Single protein structure of sequence from Erwinia chrysanthemi with , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond., Hege T, Feltzer RE, Gray RD, Baumann U, J Biol Chem. 2001 Sep 14;276(37):35087-92. Epub 2001 Jul 9. PMID:11445573
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Categories: Erwinia chrysanthemi | Single protein | Hege, T. | CA | PO4 | ZN | Hydrolase | Metalloprotease | Protease
