1gqh
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1gqh.jpg|left|200px]]<br /><applet load="1gqh" size=" | + | [[Image:1gqh.jpg|left|200px]]<br /><applet load="1gqh" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gqh, resolution 2.15Å" /> | caption="1gqh, resolution 2.15Å" /> | ||
'''QUERCETIN 2,3-DIOXYGENASE IN COMPLEX WITH THE INHIBITOR KOJIC ACID'''<br /> | '''QUERCETIN 2,3-DIOXYGENASE IN COMPLEX WITH THE INHIBITOR KOJIC ACID'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1GQH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_japonicus Aspergillus japonicus] with NAG, CU and KOJ as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Quercetin_2,3-dioxygenase Quercetin 2,3-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.24 1.13.11.24] Known structural/functional Site: <scene name='pdbsite=KOA:Man Binding Site For Chain C'>KOA</scene>. Full crystallographic information is available from [http:// | + | 1GQH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_japonicus Aspergillus japonicus] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=CU:'>CU</scene> and <scene name='pdbligand=KOJ:'>KOJ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Quercetin_2,3-dioxygenase Quercetin 2,3-dioxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.24 1.13.11.24] Known structural/functional Site: <scene name='pdbsite=KOA:Man+Binding+Site+For+Chain+C'>KOA</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GQH OCA]. |
==Reference== | ==Reference== | ||
| Line 22: | Line 22: | ||
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:41:54 2008'' |
Revision as of 07:41, 3 February 2008
|
QUERCETIN 2,3-DIOXYGENASE IN COMPLEX WITH THE INHIBITOR KOJIC ACID
Overview
The crystal structures of the copper-dependent Aspergillus japonicus, quercetin 2,3-dioxygenase (2,3QD) complexed with the inhibitors, diethyldithiocarbamate (DDC) and kojic acid (KOJ) are reported at 1.70 and, 2.15 A resolution, respectively. Both inhibitors asymmetrically chelate, the metal center and assume a common orientation in the active site cleft., Their molecular plane blocks access to the inner portion of the cavity, which is lined by the side chains of residues Met51, Thr53, Phe75, Phe114, and Met123 and which is believed to bind the flavonol B-ring of the, natural substrate. The binding of the inhibitors brings order into the, mixed coordination observed in the native enzyme. DDC and KOJ induce a, single conformation of the Glu73 side chain, although in different ways., In the presence of DDC, Glu73 is detached from the copper ion with its, carboxylate moiety pointing away from the active site cavity. In contrast, when KOJ is bound, Glu73 ligates the Cu ion through its O(epsilon)(1) atom, with a monodentate geometry. Compared to the native coordinating, conformation, this conformation is approximately 90 degrees rotated about, the chi(3) angle. This latter Glu73 conformation is compatible with the, presence of a bound substrate.
About this Structure
1GQH is a Single protein structure of sequence from Aspergillus japonicus with , and as ligands. Active as Quercetin 2,3-dioxygenase, with EC number 1.13.11.24 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Functional analysis of the copper-dependent quercetin 2,3-dioxygenase. 1. Ligand-induced coordination changes probed by X-ray crystallography: inhibition, ordering effect, and mechanistic insights., Steiner RA, Kooter IM, Dijkstra BW, Biochemistry. 2002 Jun 25;41(25):7955-62. PMID:12069585
Page seeded by OCA on Sun Feb 3 09:41:54 2008
