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1gql
From Proteopedia
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| - | [[Image:1gql.gif|left|200px]]<br /><applet load="1gql" size=" | + | [[Image:1gql.gif|left|200px]]<br /><applet load="1gql" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gql, resolution 1.67Å" /> | caption="1gql, resolution 1.67Å" /> | ||
'''STRUCTURE OF PSEUDOMONAS CELLULOSA ALPHA-D-GLUCURONIDASE COMPLEXED WITH GLUCURONIC ACID AND XYLOTRIOSE'''<br /> | '''STRUCTURE OF PSEUDOMONAS CELLULOSA ALPHA-D-GLUCURONIDASE COMPLEXED WITH GLUCURONIC ACID AND XYLOTRIOSE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus] with BDP, CO and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-glucuronidase Alpha-glucuronidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.139 3.2.1.139] Known structural/functional Site: <scene name='pdbsite=AC1:Xyp Binding Site For Chain B'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1GQL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus] with <scene name='pdbligand=BDP:'>BDP</scene>, <scene name='pdbligand=CO:'>CO</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-glucuronidase Alpha-glucuronidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.139 3.2.1.139] Known structural/functional Site: <scene name='pdbsite=AC1:Xyp+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GQL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: xylotriose]] | [[Category: xylotriose]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:41:57 2008'' |
Revision as of 07:41, 3 February 2008
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STRUCTURE OF PSEUDOMONAS CELLULOSA ALPHA-D-GLUCURONIDASE COMPLEXED WITH GLUCURONIC ACID AND XYLOTRIOSE
Overview
Alpha-glucuronidases, components of an ensemble of enzymes central to the, recycling of photosynthetic biomass, remove the alpha-1,2 linked, 4-O-methyl glucuronic acid from xylans. The structure of the, alpha-glucuronidase, GlcA67A, from Pseudomonas cellulosa reveals three, domains, the central of which is a (beta/alpha)(8) barrel housing the, catalytic apparatus. Complexes of the enzyme with the individual reaction, products, either xylobiose or glucuronic acid, and the ternary complex of, both glucuronic acid and xylotriose reveal a "blind" pocket which selects, for short decorated xylooligosaccharides substituted with the uronic acid, at their nonreducing end, consistent with kinetic data. The catalytic, center reveals a constellation of carboxylates; Glu292 is poised to, provide protonic assistance to leaving group departure with Glu393 and, Asp365 both appropriately positioned to provide base-catalyzed assistance, for inverting nucleophilic attack by water.
About this Structure
1GQL is a Single protein structure of sequence from Cellvibrio japonicus with , and as ligands. Active as Alpha-glucuronidase, with EC number 3.2.1.139 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A., Nurizzo D, Nagy T, Gilbert HJ, Davies GJ, Structure. 2002 Apr;10(4):547-56. PMID:11937059
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