1gv7
From Proteopedia
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| - | [[Image:1gv7.gif|left|200px]]<br /><applet load="1gv7" size=" | + | [[Image:1gv7.gif|left|200px]]<br /><applet load="1gv7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gv7, resolution 2.10Å" /> | caption="1gv7, resolution 2.10Å" /> | ||
'''ARH-I, AN ANGIOGENIN/RNASE A CHIMERA'''<br /> | '''ARH-I, AN ANGIOGENIN/RNASE A CHIMERA'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GV7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_bos_taurus Homo sapiens, bos taurus] with CIT as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Known structural/functional Site: <scene name='pdbsite=AC1:Cit Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1GV7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens,_bos_taurus Homo sapiens, bos taurus] with <scene name='pdbligand=CIT:'>CIT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Known structural/functional Site: <scene name='pdbsite=AC1:Cit+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GV7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: pancreatic ribonuclease]] | [[Category: pancreatic ribonuclease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:42:59 2008'' |
Revision as of 07:43, 3 February 2008
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ARH-I, AN ANGIOGENIN/RNASE A CHIMERA
Overview
Angiogenin and ribonuclease A share 33% sequence identity but have, distinct functions. Angiogenin is a potent inducer of angiogenesis that is, only weakly ribonucleolytic, whereas ribonuclease A is a robust, ribonuclease that is not angiogenic. A chimera ("ARH-I"), in which, angiogenin residues 58-70 are replaced with residues 59-73 of ribonuclease, A, has intermediate ribonucleolytic potency and no angiogenic activity., Here we report a crystal structure of ARH-I that reveals the molecular, basis for these characteristics. The ribonuclease A-derived (guest), segment adopts a structure largely similar to that in ribonuclease A, and, successfully converts this region from a cell-binding site to a, purine-binding site. At the same time, its presence causes complex changes, in the angiogenin-derived (host) portion that account for much of the, increased ribonuclease activity of ARH-I. Guest-host interactions of this, type probably occur more generally in protein chimeras, emphasizing the, importance of direct structural information for understanding the, functional behavior of such molecules.
About this Structure
1GV7 is a Single protein structure of sequence from Homo sapiens, bos taurus with as ligand. Active as Pancreatic ribonuclease, with EC number 3.1.27.5 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Guest-host crosstalk in an angiogenin-RNase A chimeric protein., Holloway DE, Shapiro R, Hares MC, Leonidas DD, Acharya KR, Biochemistry. 2002 Aug 20;41(33):10482-9. PMID:12173935
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