1gwc
From Proteopedia
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| - | [[Image:1gwc.gif|left|200px]]<br /><applet load="1gwc" size=" | + | [[Image:1gwc.gif|left|200px]]<br /><applet load="1gwc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gwc, resolution 2.25Å" /> | caption="1gwc, resolution 2.25Å" /> | ||
'''THE STRUCTURE OF A TAU CLASS GLUTATHIONE S-TRANSFERASE FROM WHEAT, ACTIVE IN HERBICIDE DETOXIFICATION'''<br /> | '''THE STRUCTURE OF A TAU CLASS GLUTATHIONE S-TRANSFERASE FROM WHEAT, ACTIVE IN HERBICIDE DETOXIFICATION'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GWC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aegilops_tauschii Aegilops tauschii] with SO4 and GTX as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain C'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1GWC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aegilops_tauschii Aegilops tauschii] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GTX:'>GTX</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Known structural/functional Site: <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+C'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWC OCA]. |
==Reference== | ==Reference== | ||
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[[Category: tau class]] | [[Category: tau class]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:43:24 2008'' |
Revision as of 07:43, 3 February 2008
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THE STRUCTURE OF A TAU CLASS GLUTATHIONE S-TRANSFERASE FROM WHEAT, ACTIVE IN HERBICIDE DETOXIFICATION
Overview
Glutathione S-transferases (GSTs) from the phi (GSTF) and tau (GSTU), classes are unique to plants and play important roles in stress tolerance, and secondary metabolism as well as catalyzing the detoxification of, herbicides in crops and weeds. We have cloned and functionally, characterized a group of GSTUs from wheat treated with, fenchlorazole-ethyl, a herbicide safener. One of these enzymes, TaGSTU4-4, was highly active in conjugating the chemically distinct wheat herbicides, fenoxaprop and dimethenamid. The structure of TaGSTU4-4 has been, determined at 2.2 A resolution in complex with S-hexylglutathione. This, enzyme is the first tau class GST structure to be determined and most, closely resembles the omega class GSTs, but without the unique N-terminal, extension or active site cysteine. The X-ray structure identifies key, amino acid residues in the hydrophobic binding site and provides insights, into the substrate specificity of these enzymes.
About this Structure
1GWC is a Single protein structure of sequence from Aegilops tauschii with and as ligands. Active as Glutathione transferase, with EC number 2.5.1.18 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure of a tau class glutathione S-transferase from wheat active in herbicide detoxification., Thom R, Cummins I, Dixon DP, Edwards R, Cole DJ, Lapthorn AJ, Biochemistry. 2002 Jun 4;41(22):7008-20. PMID:12033934
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