1gwd
From Proteopedia
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| - | [[Image:1gwd.jpg|left|200px]]<br /><applet load="1gwd" size=" | + | [[Image:1gwd.jpg|left|200px]]<br /><applet load="1gwd" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gwd, resolution 1.77Å" /> | caption="1gwd, resolution 1.77Å" /> | ||
'''TRI-IODIDE DERIVATIVE OF HEN EGG-WHITE LYSOZYME'''<br /> | '''TRI-IODIDE DERIVATIVE OF HEN EGG-WHITE LYSOZYME'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GWD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with IOD, CL, NA, EDO and CMO as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Site: <scene name='pdbsite=EA1:Egl Binding Site For Chain A'>EA1</scene>. Full crystallographic information is available from [http:// | + | 1GWD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=IOD:'>IOD</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=EDO:'>EDO</scene> and <scene name='pdbligand=CMO:'>CMO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Site: <scene name='pdbsite=EA1:Egl+Binding+Site+For+Chain+A'>EA1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:43:25 2008'' |
Revision as of 07:43, 3 February 2008
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TRI-IODIDE DERIVATIVE OF HEN EGG-WHITE LYSOZYME
Overview
A series of experiments performed at Cu Kalpha wavelength on in-house, X-ray equipment are presented which investigate two possibilities for, enhancing the experimental phasing signal by means of (i) triiodide/iodide, soaks using KI/I(2) and (ii) combinations of counter-ions introduced using, the short cryosoak method. Triiodide-derivative crystal structures for, five test proteins have been refined and reveal that iodine can bind as, polyiodide and single iodide ions through hydrophobic and hydrogen-bonding, interactions both at the molecular surface and in intramolecular and, intermolecular cavities. In three cases, the structures could be, automatically determined with autoSHARP using in-house SAD and SIRAS data., The investigation of combinatorial counter-ion replacement using multiple, salts with Na(+) and Cs(+) as cations and I(-) and Cl(-) as anions reveals, that, for the case of hen egg-white lysozyme, significant improvement in, phasing signal is obtained by the combined use of salts compared with, SIRAS methods using native and single short-soak derivative data sets.
About this Structure
1GWD is a Single protein structure of sequence from Gallus gallus with , , , and as ligands. Active as Lysozyme, with EC number 3.2.1.17 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Triiodide derivatization and combinatorial counter-ion replacement: two methods for enhancing phasing signal using laboratory Cu Kalpha X-ray equipment., Evans G, Bricogne G, Acta Crystallogr D Biol Crystallogr. 2002 Jun;58(Pt 6 Pt 2):976-91. Epub, 2002 May 29. PMID:12037300
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Categories: Gallus gallus | Lysozyme | Single protein | Bricogne, G. | Evans, G. | CL | CMO | EDO | IOD | NA | Aller | Bacteriolytic enzyme | Glycosidase | Hydrolase | Signal
