2bz4
From Proteopedia
(New page: 200px<br /> <applet load="2bz4" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bz4, resolution 1.86Å" /> '''STRUCTURE OF E. COL...) |
|||
| Line 8: | Line 8: | ||
==About this Structure== | ==About this Structure== | ||
| - | 2BZ4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with NH4 and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BZ4 OCA]]. | + | 2BZ4 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with NH4 and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Beta-ketoacyl-acyl-carrier-protein_synthase_I Beta-ketoacyl-acyl-carrier-protein synthase I]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.41 2.3.1.41]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BZ4 OCA]]. |
==Reference== | ==Reference== | ||
Fatty acid synthesis. Role of active site histidines and lysine in Cys-His-His-type beta-ketoacyl-acyl carrier protein synthases., von Wettstein-Knowles P, Olsen JG, McGuire KA, Henriksen A, FEBS J. 2006 Feb;273(4):695-710. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16441657 16441657] | Fatty acid synthesis. Role of active site histidines and lysine in Cys-His-His-type beta-ketoacyl-acyl carrier protein synthases., von Wettstein-Knowles P, Olsen JG, McGuire KA, Henriksen A, FEBS J. 2006 Feb;273(4):695-710. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16441657 16441657] | ||
| + | [[Category: Beta-ketoacyl-acyl-carrier-protein synthase I]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 26: | Line 27: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:30:57 2007'' |
Revision as of 10:26, 30 October 2007
|
STRUCTURE OF E. COLI KAS I H298Q MUTANT
Overview
Beta-ketoacyl-acyl carrier protein (ACP) synthase enzymes join short, carbon units to construct fatty acyl chains by a three-step Claisen, condensation reaction. The reaction starts with a trans thioesterification, of the acyl primer substrate from ACP to the enzyme. Subsequently, the, donor substrate malonyl-ACP is decarboxylated to form a carbanion, intermediate, which in the third step attacks C1 of the primer substrate, giving rise to an elongated acyl chain. A subgroup of beta-ketoacyl-ACP, synthases, including mitochondrial beta-ketoacyl-ACP synthase, bacterial, plus plastid beta-ketoacyl-ACP synthases I and II, and a domain of human, fatty acid synthase, have a Cys-His-His triad and also a completely, conserved Lys in the active site. To examine the role of these residues in, ... [(full description)]
About this Structure
2BZ4 is a [Single protein] structure of sequence from [Escherichia coli] with NH4 and SO4 as [ligands]. Active as [Beta-ketoacyl-acyl-carrier-protein synthase I], with EC number [2.3.1.41]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Fatty acid synthesis. Role of active site histidines and lysine in Cys-His-His-type beta-ketoacyl-acyl carrier protein synthases., von Wettstein-Knowles P, Olsen JG, McGuire KA, Henriksen A, FEBS J. 2006 Feb;273(4):695-710. PMID:16441657
Page seeded by OCA on Tue Oct 30 12:30:57 2007
