1gz3
From Proteopedia
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| - | [[Image:1gz3.jpg|left|200px]]<br /><applet load="1gz3" size=" | + | [[Image:1gz3.jpg|left|200px]]<br /><applet load="1gz3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1gz3, resolution 2.3Å" /> | caption="1gz3, resolution 2.3Å" /> | ||
'''MOLECULAR MECHANISM FOR THE REGULATION OF HUMAN MITOCHONDRIAL NAD(P)+-DEPENDENT MALIC ENZYME BY ATP AND FUMARATE'''<br /> | '''MOLECULAR MECHANISM FOR THE REGULATION OF HUMAN MITOCHONDRIAL NAD(P)+-DEPENDENT MALIC ENZYME BY ATP AND FUMARATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GZ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with OXL, MN, ATP and FUM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase_(oxaloacetate-decarboxylating) Malate dehydrogenase (oxaloacetate-decarboxylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.38 1.1.1.38] Known structural/functional Site: <scene name='pdbsite=AC1:Fum Binding Site For Chain D'>AC1</scene>. Full crystallographic information is available from [http:// | + | 1GZ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=OXL:'>OXL</scene>, <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=ATP:'>ATP</scene> and <scene name='pdbligand=FUM:'>FUM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_dehydrogenase_(oxaloacetate-decarboxylating) Malate dehydrogenase (oxaloacetate-decarboxylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.38 1.1.1.38] Known structural/functional Site: <scene name='pdbsite=AC1:Fum+Binding+Site+For+Chain+D'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: protein structure]] | [[Category: protein structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:44:11 2008'' |
Revision as of 07:44, 3 February 2008
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MOLECULAR MECHANISM FOR THE REGULATION OF HUMAN MITOCHONDRIAL NAD(P)+-DEPENDENT MALIC ENZYME BY ATP AND FUMARATE
Contents |
Overview
The regulation of human mitochondrial NAD(P)+-dependent malic enzyme, (m-NAD-ME) by ATP and fumarate may be crucial for the metabolism of, glutamine for energy production in rapidly proliferating tissues and, tumors. Here we report the crystal structure at 2.2 A resolution of, m-NAD-ME in complex with ATP, Mn2+, tartronate, and fumarate. Our, structural, kinetic, and mutagenesis studies reveal unexpectedly that ATP, is an active-site inhibitor of the enzyme, despite the presence of an exo, binding site. The structure also reveals the allosteric binding site for, fumarate in the dimer interface. Mutations in this binding site abolished, the activating effects of fumarate. Comparison to the structure in the, absence of fumarate indicates a possible molecular mechanism for the, allosteric function of this compound.
Disease
Known disease associated with this structure: Epilepsy, idopathic generalized, susceptibility to OMIM:[154270]
About this Structure
1GZ3 is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Active as Malate dehydrogenase (oxaloacetate-decarboxylating), with EC number 1.1.1.38 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate., Yang Z, Lanks CW, Tong L, Structure. 2002 Jul;10(7):951-60. PMID:12121650
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