1bzf
From Proteopedia
(New page: 200px<br /> <applet load="1bzf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bzf" /> '''NMR SOLUTION STRUCTURE AND DYNAMICS OF THE ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1BZF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei]] with TMQ as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BZF OCA]]. | + | 1BZF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Lactobacillus_casei Lactobacillus casei]] with TMQ as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Dihydrofolate_reductase Dihydrofolate reductase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.3 1.5.1.3]]. Structure known Active Site: TMQ. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BZF OCA]]. |
==Reference== | ==Reference== | ||
Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate., Polshakov VI, Birdsall B, Frenkiel TA, Gargaro AR, Feeney J, Protein Sci. 1999 Mar;8(3):467-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10091649 10091649] | Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate., Polshakov VI, Birdsall B, Frenkiel TA, Gargaro AR, Feeney J, Protein Sci. 1999 Mar;8(3):467-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10091649 10091649] | ||
| + | [[Category: Dihydrofolate reductase]] | ||
[[Category: Lactobacillus casei]] | [[Category: Lactobacillus casei]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:31:10 2007'' |
Revision as of 10:26, 30 October 2007
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NMR SOLUTION STRUCTURE AND DYNAMICS OF THE COMPLEX OF LACTOBACILLUS CASEI DIHYDROFOLATE REDUCTASE WITH THE NEW LIPOPHILIC ANTIFOLATE DRUG TRIMETREXATE, 22 STRUCTURES
Overview
We have determined the three-dimensional solution structure of the complex, of Lactobacillus casei dihydrofolate reductase and the anticancer drug, trimetrexate. Two thousand seventy distance, 345 dihedral angle, and 144, hydrogen bond restraints were obtained from analysis of multidimensional, NMR spectra recorded for complexes containing 15N-labeled protein., Simulated annealing calculations produced a family of 22 structures fully, consistent with the constraints. Several intermolecular protein-ligand, NOEs were obtained by using a novel approach monitoring temperature, effects of NOE signals resulting from dynamic processes in the bound, ligand. At low temperature (5 degrees C) the trimethoxy ring of bound, trimetrexate is flipping sufficiently slowly to give narrow signals in, slow ... [(full description)]
About this Structure
1BZF is a [Single protein] structure of sequence from [Lactobacillus casei] with TMQ as [ligand]. Active as [Dihydrofolate reductase], with EC number [1.5.1.3]. Structure known Active Site: TMQ. Full crystallographic information is available from [OCA].
Reference
Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate., Polshakov VI, Birdsall B, Frenkiel TA, Gargaro AR, Feeney J, Protein Sci. 1999 Mar;8(3):467-81. PMID:10091649
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