1h0a
From Proteopedia
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| - | [[Image:1h0a.jpg|left|200px]]<br /><applet load="1h0a" size=" | + | [[Image:1h0a.jpg|left|200px]]<br /><applet load="1h0a" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h0a, resolution 1.70Å" /> | caption="1h0a, resolution 1.70Å" /> | ||
'''EPSIN ENTH BOUND TO INS(1,4,5)P3'''<br /> | '''EPSIN ENTH BOUND TO INS(1,4,5)P3'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H0A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with DIO and I3P as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=DI1:I3p Binding Site For Chain A'>DI1</scene>. Full crystallographic information is available from [http:// | + | 1H0A is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=DIO:'>DIO</scene> and <scene name='pdbligand=I3P:'>I3P</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=DI1:I3p+Binding+Site+For+Chain+A'>DI1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H0A OCA]. |
==Reference== | ==Reference== | ||
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[[Category: triskelion]] | [[Category: triskelion]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:44:42 2008'' |
Revision as of 07:44, 3 February 2008
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EPSIN ENTH BOUND TO INS(1,4,5)P3
Overview
Clathrin-mediated endocytosis involves cargo selection and membrane, budding into vesicles with the aid of a protein coat. Formation of, invaginated pits on the plasma membrane and subsequent budding of vesicles, is an energetically demanding process that involves the cooperation of, clathrin with many different proteins. Here we investigate the role of the, brain-enriched protein epsin 1 in this process. Epsin is targeted to areas, of endocytosis by binding the membrane lipid, phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)). We show here that, epsin 1 directly modifies membrane curvature on binding to PtdIns(4,5)P(2), in conjunction with clathrin polymerization. We have discovered that, formation of an amphipathic alpha-helix in epsin is coupled to, PtdIns(4,5)P(2) binding. Mutation of residues on the hydrophobic region of, this helix abolishes the ability to curve membranes. We propose that this, helix is inserted into one leaflet of the lipid bilayer, inducing, curvature. On lipid monolayers epsin alone is sufficient to facilitate the, formation of clathrin-coated invaginations.
About this Structure
1H0A is a Single protein structure of sequence from Rattus norvegicus with and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Curvature of clathrin-coated pits driven by epsin., Ford MG, Mills IG, Peter BJ, Vallis Y, Praefcke GJ, Evans PR, McMahon HT, Nature. 2002 Sep 26;419(6905):361-6. PMID:12353027
Page seeded by OCA on Sun Feb 3 09:44:42 2008
Categories: Rattus norvegicus | Single protein | Evans, P.R. | Ford, M.G.J. | Mcmahon, H.T. | DIO | I3P | 4 | 5)p3 | Alpha-alpha superhelix | Clathrin | Coated vesicles | Endocytosis | Enth | Epsin | Ins(1 | Triskelion
