1h1y
From Proteopedia
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| - | [[Image:1h1y.gif|left|200px]]<br /><applet load="1h1y" size=" | + | [[Image:1h1y.gif|left|200px]]<br /><applet load="1h1y" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h1y, resolution 1.87Å" /> | caption="1h1y, resolution 1.87Å" /> | ||
'''THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE'''<br /> | '''THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H1Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribulose-phosphate_3-epimerase Ribulose-phosphate 3-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.1 5.1.3.1] Known structural/functional Site: <scene name='pdbsite=SO1:So4 Binding Site For Chain B'>SO1</scene>. Full crystallographic information is available from [http:// | + | 1H1Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Ribulose-phosphate_3-epimerase Ribulose-phosphate 3-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.1 5.1.3.1] Known structural/functional Site: <scene name='pdbsite=SO1:So4+Binding+Site+For+Chain+B'>SO1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H1Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: oxidative pentose phosphate pathway]] | [[Category: oxidative pentose phosphate pathway]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:45:18 2008'' |
Revision as of 07:45, 3 February 2008
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THE STRUCTURE OF THE CYTOSOLIC D-RIBULOSE-5-PHOSPHATE 3-EPIMERASE FROM RICE COMPLEXED WITH SULFATE
Overview
Cytosolic D-ribulose-5-phosphate 3-epimerase from rice was crystallized, after EDTA treatment and structurally elucidated by X-ray diffraction to, 1.9A resolution. A prominent Zn(2+) site at the active center was, established in a soaking experiment. The structure was compared with that, of the EDTA-treated crystalline enzyme from the chloroplasts of potato, plant leaves showing some structural differences, in particular the, "closed" state of a strongly conserved mobile loop covering the substrate, at its putative binding site. The previous proposal for the active center, was confirmed and the most likely substrate binding position and, conformation was derived from the locations of the bound zinc and sulfate, ions and of three water molecules. Assuming that the bound zinc ion is an, integral part of the enzyme, a reaction mechanism involving a, well-stabilized cis-enediolate intermediate is suggested.
About this Structure
1H1Y is a Single protein structure of sequence from Oryza sativa with as ligand. Active as Ribulose-phosphate 3-epimerase, with EC number 5.1.3.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structure and catalytic mechanism of the cytosolic D-ribulose-5-phosphate 3-epimerase from rice., Jelakovic S, Kopriva S, Suss KH, Schulz GE, J Mol Biol. 2003 Feb 7;326(1):127-35. PMID:12547196
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