1h2t
From Proteopedia
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| - | [[Image:1h2t.gif|left|200px]]<br /><applet load="1h2t" size=" | + | [[Image:1h2t.gif|left|200px]]<br /><applet load="1h2t" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h2t, resolution 2.10Å" /> | caption="1h2t, resolution 2.10Å" /> | ||
'''STRUCTURE OF THE HUMAN NUCLEAR CAP-BINDING-COMPLEX (CBC) IN COMPLEX WITH A CAP ANALOGUE M7GPPPG'''<br /> | '''STRUCTURE OF THE HUMAN NUCLEAR CAP-BINDING-COMPLEX (CBC) IN COMPLEX WITH A CAP ANALOGUE M7GPPPG'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GDP as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=CAP:M7gpppg Binding Site For Chain Z'>CAP</scene>. Full crystallographic information is available from [http:// | + | 1H2T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=CAP:M7gpppg+Binding+Site+For+Chain+Z'>CAP</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rnp domain]] | [[Category: rnp domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:45:34 2008'' |
Revision as of 07:45, 3 February 2008
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STRUCTURE OF THE HUMAN NUCLEAR CAP-BINDING-COMPLEX (CBC) IN COMPLEX WITH A CAP ANALOGUE M7GPPPG
Overview
The heterodimeric nuclear cap-binding complex (CBC) binds to the 5' cap, structure of RNAs in the nucleus and plays a central role in their diverse, maturation steps. We describe the crystal structure at 2.1 A resolution of, human CBC bound to an m(7)GpppG cap analogue. Comparison with the, structure of uncomplexed CBC shows that cap binding induces co-operative, folding around the dinucleotide of some 50 residues from the N- and, C-terminal extensions to the central RNP domain of the small subunit, CBP20. The cap-bound conformation of CBP20 is stabilized by an intricate, network of interactions both to the ligand and within the subunit, as well, as new interactions of the CBP20 N-terminal tail with the large subunit, CBP80. Although the structure is very different from that of other known, cap-binding proteins, such as the cytoplasmic cap-binding protein eIF4E, specificity for the methylated guanosine again is achieved by sandwiching, the base between two aromatic residues, in this case two conserved, tyrosines. Implications for the transfer of capped mRNAs to eIF4E, required for translation initiation, are discussed.
About this Structure
1H2T is a Protein complex structure of sequences from Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Large-scale induced fit recognition of an m(7)GpppG cap analogue by the human nuclear cap-binding complex., Mazza C, Segref A, Mattaj IW, Cusack S, EMBO J. 2002 Oct 15;21(20):5548-57. PMID:12374755
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