1h4i
From Proteopedia
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| - | [[Image:1h4i.gif|left|200px]]<br /><applet load="1h4i" size=" | + | [[Image:1h4i.gif|left|200px]]<br /><applet load="1h4i" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h4i, resolution 1.94Å" /> | caption="1h4i, resolution 1.94Å" /> | ||
'''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE'''<br /> | '''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H4I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens] with CA and PQQ as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8] Known structural/functional Sites: <scene name='pdbsite=AC1:Pqq Binding Site For Chain A'>AC1</scene>, <scene name='pdbsite=AC2:Ca Binding Site For Chain A'>AC2</scene>, <scene name='pdbsite=AC3:Pqq Binding Site For Chain C'>AC3</scene> and <scene name='pdbsite=AC4:Ca Binding Site For Chain C'>AC4</scene>. Full crystallographic information is available from [http:// | + | 1H4I is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=PQQ:'>PQQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(acceptor) Alcohol dehydrogenase (acceptor)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8] Known structural/functional Sites: <scene name='pdbsite=AC1:Pqq+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Ca+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Pqq+Binding+Site+For+Chain+C'>AC3</scene> and <scene name='pdbsite=AC4:Ca+Binding+Site+For+Chain+C'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: quinoprotein]] | [[Category: quinoprotein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:46:15 2008'' |
Revision as of 07:46, 3 February 2008
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METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE
Overview
BACKGROUND: Methanol dehydrogenase (MDH) is a bacterial periplasmic, quinoprotein; it has pyrrolo-quinoline quinone (PQQ) as its prosthetic, group, requires Ca2+ for activity and uses cytochrome cL as its electron, acceptor. Low-resolution structures of MDH have already been determined., RESULTS: The structure of the alpha 2 beta 2 tetramer of MDH from, Methylobacterium extorquens has now been determined at 1.94 A with an, R-factor of 19.85%. CONCLUSIONS: The alpha-subunit of MDH has an, eight-fold radial symmetry, with its eight beta-sheets stabilized by a, novel tryptophan docking motif. The PQQ in the active site is held in, place by a coplanar tryptophan and by a novel disulphide ring formed, between adjacent cysteines which are bonded by an unusual non-planar trans, peptide bond. One of the carbonyl oxygens of PQQ is bonded to the Ca2+, probably facilitating attack on the substrate, and the other carbonyl, oxygen is out of the plane of the ring, confirming the presence of the, predicted free-radical semiquinone form of the prosthetic group.
About this Structure
1H4I is a Protein complex structure of sequences from Methylobacterium extorquens with and as ligands. Active as Alcohol dehydrogenase (acceptor), with EC number 1.1.99.8 Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
The refined structure of the quinoprotein methanol dehydrogenase from Methylobacterium extorquens at 1.94 A., Ghosh M, Anthony C, Harlos K, Goodwin MG, Blake C, Structure. 1995 Feb 15;3(2):177-87. PMID:7735834
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