1h68
From Proteopedia
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| - | [[Image:1h68.gif|left|200px]]<br /><applet load="1h68" size=" | + | [[Image:1h68.gif|left|200px]]<br /><applet load="1h68" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1h68, resolution 2.1Å" /> | caption="1h68, resolution 2.1Å" /> | ||
'''SENSORY RHODOPSIN II'''<br /> | '''SENSORY RHODOPSIN II'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H68 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis] with CL, LEU and RET as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1H68 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb27_1.html Bacteriorhodopsin]]. Known structural/functional Sites: <scene name='pdbsite=CL:Cl Binding Site For Chain A'>CL</scene> and <scene name='pdbsite=RET:Ret Binding Site For Chain A'>RET</scene>. Full crystallographic information is available from [http:// | + | 1H68 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Natronomonas_pharaonis Natronomonas pharaonis] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=LEU:'>LEU</scene> and <scene name='pdbligand=RET:'>RET</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1H68 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb27_1.html Bacteriorhodopsin]]. Known structural/functional Sites: <scene name='pdbsite=CL:Cl+Binding+Site+For+Chain+A'>CL</scene> and <scene name='pdbsite=RET:Ret+Binding+Site+For+Chain+A'>RET</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H68 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: phototaxis]] | [[Category: phototaxis]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:46:59 2008'' |
Revision as of 07:46, 3 February 2008
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SENSORY RHODOPSIN II
Overview
Sensory rhodopsins (SRs) belong to a subfamily of heptahelical, transmembrane proteins containing a retinal chromophore. These, photoreceptors mediate the cascade of vision in animal eyes and phototaxis, in archaebacteria and unicellular flagellated algae. Signal transduction, by these photoreceptors occurs by means of transducer proteins. The two, archaebacterial sensory rhodopsins SRI and SRII are coupled to the, membrane-bound HtrI and HtrII transducer proteins. Activation of these, proteins initiates phosphorylation cascades that modulate the flagellar, motors, resulting in either attractant (SRI) or repellent (SRII), phototaxis. In addition, transducer-free SRI and SRII were shown to, operate as proton pumps, analogous to bacteriorhodopsin. Here, we present, the x-ray structure of SRII from Natronobacterium pharaonis (pSRII) at, 2.1-A resolution, revealing a unique molecular architecture of the, retinal-binding pocket. In particular, the structure of pSRII exhibits a, largely unbent conformation of the retinal (as compared with, bacteriorhodopsin and halorhodopsin), a hydroxyl group of Thr-204 in the, vicinity of the Schiff base, and an outward orientation of the guanidinium, group of Arg-72. Furthermore, the structure reveals a putative chloride, ion that is coupled to the Schiff base by means of a hydrogen-bond network, and a unique, positively charged surface patch for a probable interaction, with HtrII. The high-resolution structure of pSRII provides a structural, basis to elucidate the mechanisms of phototransduction and color tuning.
About this Structure
1H68 is a Single protein structure of sequence from Natronomonas pharaonis with , and as ligands. The following page contains interesting information on the relation of 1H68 with [Bacteriorhodopsin]. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
X-ray structure of sensory rhodopsin II at 2.1-A resolution., Royant A, Nollert P, Edman K, Neutze R, Landau EM, Pebay-Peyroula E, Navarro J, Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10131-6. Epub 2001 Aug 14. PMID:11504917
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