1qje

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(New page: 200px<br /> <applet load="1qje" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qje, resolution 1.35&Aring;" /> '''ISOPENICILLIN N SYN...)
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==About this Structure==
==About this Structure==
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1QJE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with SO4, FE2 and IP1 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QJE OCA]].
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1QJE is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]] with SO4, FE2 and IP1 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: SA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QJE OCA]].
==Reference==
==Reference==
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[[Category: penicillin biosynthesis]]
[[Category: penicillin biosynthesis]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:24:36 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:32:45 2007''

Revision as of 10:28, 30 October 2007


1qje, resolution 1.35Å

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ISOPENICILLIN N SYNTHASE FROM ASPERGILLUS NIDULANS (IP1-FE COMPLEX)

Overview

Isopenicillin N synthase (IPNS), a non-haem iron-dependent oxidase, catalyses the biosynthesis of isopenicillin N (IPN), the precursor of all, penicillins and cephalosporins. The key steps in this reaction are the two, iron-dioxygen-mediated ring closures of the tripeptide, delta-(L-alpha-aminoadipoyl)-L-cysteinyl-D-valine (ACV). It has been, proposed that the four-membered beta-lactam ring forms initially, associated with a highly oxidized iron(iv)-oxo (ferryl) moiety, which, subsequently mediates closure of the five-membered thiazolidine ring. Here, we describe observation of the IPNS reaction in crystals by X-ray, crystallography. IPNS Fe2+ substrate crystals were grown anaerobically, exposed to high pressures of oxygen to promote reaction and frozen, and, their structures were ... [(full description)]

About this Structure

1QJE is a [Single protein] structure of sequence from [Emericella nidulans] with SO4, FE2 and IP1 as [ligands]. Structure known Active Site: SA. Full crystallographic information is available from [OCA].

Reference

The reaction cycle of isopenicillin N synthase observed by X-ray diffraction., Burzlaff NI, Rutledge PJ, Clifton IJ, Hensgens CM, Pickford M, Adlington RM, Roach PL, Baldwin JE, Nature. 1999 Oct 14;401(6754):721-4. PMID:10537113

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