1hkc

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[[Image:1hkc.gif|left|200px]]<br /><applet load="1hkc" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1hkc.gif|left|200px]]<br /><applet load="1hkc" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1hkc, resolution 2.80&Aring;" />
caption="1hkc, resolution 2.80&Aring;" />
'''RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE'''<br />
'''RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE'''<br />
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==About this Structure==
==About this Structure==
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1HKC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> and <scene name='pdbligand=K:POTASSIUM ION'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] Known structural/functional Sites: <scene name='pdbsite=GLN:Glc Binding Site In N-Terminal Domain'>GLN</scene>, <scene name='pdbsite=MEC:Metal Ion Binding Site In C-Terminal Domain'>MEC</scene>, <scene name='pdbsite=MEN:Metal Ion Binding Site In N-Terminal Domain'>MEN</scene>, <scene name='pdbsite=PIC:Phosphate Binding Site In C-Terminal Domain'>PIC</scene> and <scene name='pdbsite=PIN:Phosphate Binding Site In N-Terminal Domain'>PIN</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HKC OCA].
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1HKC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=GLC:'>GLC</scene>, <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=K:'>K</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] Known structural/functional Sites: <scene name='pdbsite=GLN:Glc+Binding+Site+In+N-Terminal+Domain'>GLN</scene>, <scene name='pdbsite=MEC:Metal+Ion+Binding+Site+In+C-Terminal+Domain'>MEC</scene>, <scene name='pdbsite=MEN:Metal+Ion+Binding+Site+In+N-Terminal+Domain'>MEN</scene>, <scene name='pdbsite=PIC:Phosphate+Binding+Site+In+C-Terminal+Domain'>PIC</scene> and <scene name='pdbsite=PIN:Phosphate+Binding+Site+In+N-Terminal+Domain'>PIN</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKC OCA].
==Reference==
==Reference==
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[[Category: phosphotransferase]]
[[Category: phosphotransferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Dec 19 13:19:46 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:49:50 2008''

Revision as of 07:49, 3 February 2008

Image:1hkc.gif

1hkc, resolution 2.80Å

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RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE

Contents

Overview

Hexokinase I, the pacemaker of glycolysis in brain tissue and red blood, cells, is comprised of two similar domains fused into a single polypeptide, chain. The C-terminal half of hexokinase I is catalytically active, whereas the N-terminal half is necessary for the relief of product, inhibition by phosphate. A crystalline complex of recombinant human, hexokinase I with glucose and phosphate (2.8 A resolution) reveals a, single binding site for phosphate and glucose at the N-terminal half of, the enzyme. Glucose and phosphate stabilize the N-terminal half in a, closed conformation. Unexpectedly, glucose binds weakly to the C-terminal, half of the enzyme and does not by itself stabilize a closed conformation., Evidently a stable, closed C-terminal half requires either ATP or glucose, 6-phosphate along with glucose. The crystal structure here, in conjunction, with other studies in crystallography and directed mutation, puts the, phosphate regulatory site at the N-terminal half, the site of potent, product inhibition at the C-terminal half, and a secondary site for the, weak interaction of glucose 6-phosphate at the N-terminal half of the, enzyme. The relevance of crystal structures of hexokinase I to the, properties of monomeric hexokinase I and oligomers of hexokinase I bound, to the surface of mitochondria is discussed.

Disease

Known disease associated with this structure: Hemolytic anemia due to hexokinase deficiency OMIM:[142600]

About this Structure

1HKC is a Single protein structure of sequence from Homo sapiens with , and as ligands. Active as Hexokinase, with EC number 2.7.1.1 Known structural/functional Sites: , , , and . Full crystallographic information is available from OCA.

Reference

Regulation of hexokinase I: crystal structure of recombinant human brain hexokinase complexed with glucose and phosphate., Aleshin AE, Zeng C, Bartunik HD, Fromm HJ, Honzatko RB, J Mol Biol. 1998 Sep 18;282(2):345-57. PMID:9735292

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