This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1js4
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1js4.gif|left|200px]]<br /><applet load="1js4" size=" | + | [[Image:1js4.gif|left|200px]]<br /><applet load="1js4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1js4, resolution 2.0Å" /> | caption="1js4, resolution 2.0Å" /> | ||
'''ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA'''<br /> | '''ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA'''<br /> | ||
| Line 7: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1JS4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] with GLC and CA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=AA1:Active Site'>AA1</scene>, <scene name='pdbsite=AB1:Active Site'>AB1</scene>, <scene name='pdbsite=CA1:Ca Binding Site'>CA1</scene>, <scene name='pdbsite=CA2:Ca Binding Site'>CA2</scene>, <scene name='pdbsite=CA3:Ca Binding Site'>CA3</scene> and <scene name='pdbsite=CA4:Ca Binding Site'>CA4</scene>. Full crystallographic information is available from [http:// | + | 1JS4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermobifida_fusca Thermobifida fusca] with <scene name='pdbligand=GLC:'>GLC</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] Known structural/functional Sites: <scene name='pdbsite=AA1:Active+Site'>AA1</scene>, <scene name='pdbsite=AB1:Active+Site'>AB1</scene>, <scene name='pdbsite=CA1:Ca+Binding+Site'>CA1</scene>, <scene name='pdbsite=CA2:Ca+Binding+Site'>CA2</scene>, <scene name='pdbsite=CA3:Ca+Binding+Site'>CA3</scene> and <scene name='pdbsite=CA4:Ca+Binding+Site'>CA4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JS4 OCA]. |
==Reference== | ==Reference== | ||
| Line 21: | Line 21: | ||
[[Category: glycosyl hydrolase]] | [[Category: glycosyl hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:51:04 2008'' |
Revision as of 07:51, 3 February 2008
|
ENDO/EXOCELLULASE:CELLOBIOSE FROM THERMOMONOSPORA
Overview
Cellulase E4 from Thermomonospora fusca is unusual in that it has, characteristics of both exo- and endo-cellulases. Here we report the, crystal structure of a 68K M(r) fragment of E4 (E4-68) at 1.9 A, resolution. E4-68 contains both a family 9 catalytic domain, exhibiting an, (alpha/alpha)6 barrel fold, and a family III cellulose binding domain, having an antiparallel beta-sandwich fold. While neither of these folds is, novel, E4-68 provides the first cellulase structure having interacting, catalytic and cellulose binding domains. The complexes of E4-68 with, cellopentaose, cellotriose and cellobiose reveal conformational changes, associated with ligand binding and allow us to propose a catalytic, mechanism for family 9 enzymes. We also provide evidence that E4 has two, novel characteristics: first it combines exo- and endo-activities and, second, when it functions as an exo-cellulase, it cleaves off, cellotetraose units.
About this Structure
1JS4 is a Single protein structure of sequence from Thermobifida fusca with and as ligands. Active as Cellulase, with EC number 3.2.1.4 Known structural/functional Sites: , , , , and . Full crystallographic information is available from OCA.
Reference
Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca., Sakon J, Irwin D, Wilson DB, Karplus PA, Nat Struct Biol. 1997 Oct;4(10):810-8. PMID:9334746
Page seeded by OCA on Sun Feb 3 09:51:04 2008
