1lb3
From Proteopedia
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| - | [[Image:1lb3.gif|left|200px]]<br /><applet load="1lb3" size=" | + | [[Image:1lb3.gif|left|200px]]<br /><applet load="1lb3" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1lb3, resolution 1.21Å" /> | caption="1lb3, resolution 1.21Å" /> | ||
'''Structure of recombinant mouse L chain ferritin at 1.2 A resolution'''<br /> | '''Structure of recombinant mouse L chain ferritin at 1.2 A resolution'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1LB3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with SO4, CD and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=NCL:Putative Fe Core Nucleation Site In Ferritins. Ligands O ...'>NCL</scene>, <scene name='pdbsite=SS1:Intermolecular Crystal Contact. This Cd Bridge Links Two ...'>SS1</scene>, <scene name='pdbsite=SS2:These Sites Are Located At The Three-Fold Symmetry Axis ...'>SS2</scene> and <scene name='pdbsite=SS3:This Site Is Located Half Way Between The Three-Fold Axi ...'>SS3</scene>. Full crystallographic information is available from [http:// | + | 1LB3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=CD:'>CD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=NCL:Putative+Fe+Core+Nucleation+Site+In+Ferritins.+Ligands+O+...'>NCL</scene>, <scene name='pdbsite=SS1:Intermolecular+Crystal+Contact.+This+Cd+Bridge+Links+Two+...'>SS1</scene>, <scene name='pdbsite=SS2:These+Sites+Are+Located+At+The+Three-Fold+Symmetry+Axis+...'>SS2</scene> and <scene name='pdbsite=SS3:This+Site+Is+Located+Half+Way+Between+The+Three-Fold+Axi+...'>SS3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB3 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: iron storage]] | [[Category: iron storage]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:53:12 2008'' |
Revision as of 07:53, 3 February 2008
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Structure of recombinant mouse L chain ferritin at 1.2 A resolution
Overview
The first ferritin structure refined at the atomic level has been achieved, on recombinant mouse L-chain apoferritin (rMoLF) crystals. These latter, diffract to 1.2 A resolution under cryogenic conditions. When cryo-cooling, the sample, the thermal disorder usually observed at room temperature is, reduced and the low-temperature structure reveals several details, concerning the protein putative active sites and their properties. Within, the pores built up by the molecular three-fold symmetry axes, the iron, entry route to the ferritin cavity, residues H118, D131 and E134, exhibit, alternate conformations associated with the binding of partially hydrated, cadmium ions, a metal used as a crystallization agent. At the mineral, ferrihydrite nucleation center, the electron density maps evidence the, orientation of E57, E60, E61 and E64 glutamate side chains (whereas they, were observed highly disordered in previous ferritin structures determined, at room temperature) and allow a description of the site taking into, account the binding geometry of four Cd(2+) ions. Moreover, the side chain, of residue K140, lying in the vicinity of the ferrihydrite nucleation, center, is shown to interact with residue E61. As previously highlighted, this observation confirms the importance of K140 in the rMoLF sequence, as, being responsible for the low level of iron incorporation by mousel, L-chain ferritin compared to human L-chain ferritin. Finally, the, diffusion of small molecules within the ferritin cavity is illustrated, here by the presence of ordered molecules of glycerol used as a, cryo-protectant, which bind the inner cavity surface of the protein.
About this Structure
1LB3 is a Single protein structure of sequence from Mus musculus with , and as ligands. Known structural/functional Sites: , , and . Full crystallographic information is available from OCA.
Reference
Structural description of the active sites of mouse L-chain ferritin at 1.2 A resolution., Granier T, Langlois d'Estaintot B, Gallois B, Chevalier JM, Precigoux G, Santambrogio P, Arosio P, J Biol Inorg Chem. 2003 Jan;8(1-2):105-11. Epub 2002 Sep 6. PMID:12459904
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