1ak8
From Proteopedia
(New page: 200px<br /> <applet load="1ak8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ak8" /> '''NMR SOLUTION STRUCTURE OF CERIUM-LOADED CAL...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1AK8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with CE as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AK8 OCA]]. | + | 1AK8 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]] with CE as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Sites: EF1 and EF2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AK8 OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: cerium-loaded]] | [[Category: cerium-loaded]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:33:47 2007'' |
Revision as of 10:29, 30 October 2007
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NMR SOLUTION STRUCTURE OF CERIUM-LOADED CALMODULIN AMINO-TERMINAL DOMAIN (CE2-TR1C), 23 STRUCTURES
Overview
The solution structure of the dicerium(III) complex of the N-terminal, domain of calmodulin (Ce2-TR1C hereafter) has been solved employing, paramagnetic T1 relaxation enhancements and pseudocontact shifts, introduced by the Ce3+ ions, together with conventional NOE constraints., The use of pseudocontact shift constraints constitutes the first attempt, to locate metal ions within a protein structure by NMR. Like calcium(II), paramagnetic cerium(III) has been found to bind to the two metal binding, sites of the TR1C fragment of calmodulin in a cooperative manner. Due to, the presence of pseudocontact interactions between the Ce3+ ions and, protons of the 76-residue protein, the 1H NMR spectra of the complex show, resonances shifted between +22 and -9 ppm. Eighty percent of its proton, ... [(full description)]
About this Structure
1AK8 is a [Single protein] structure of sequence from [Bos taurus] with CE as [ligand]. Structure known Active Sites: EF1 and EF2. Full crystallographic information is available from [OCA].
Reference
Solution structure of the paramagnetic complex of the N-terminal domain of calmodulin with two Ce3+ ions by 1H NMR., Bentrop D, Bertini I, Cremonini MA, Forsen S, Luchinat C, Malmendal A, Biochemistry. 1997 Sep 30;36(39):11605-18. PMID:9305950
Page seeded by OCA on Tue Oct 30 12:33:47 2007
