1nvg
From Proteopedia
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| - | [[Image:1nvg.gif|left|200px]]<br /><applet load="1nvg" size=" | + | [[Image:1nvg.gif|left|200px]]<br /><applet load="1nvg" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1nvg, resolution 2.50Å" /> | caption="1nvg, resolution 2.50Å" /> | ||
'''N249Y MUTANT OF THE ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS-TETRAGONAL CRYSTAL FORM'''<br /> | '''N249Y MUTANT OF THE ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS-TETRAGONAL CRYSTAL FORM'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1NVG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] Known structural/functional Site: <scene name='pdbsite=ZNS:Residues That Bind The Catalytic Zn Ion (Zn A 500)'>ZNS</scene>. Full crystallographic information is available from [http:// | + | 1NVG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] Known structural/functional Site: <scene name='pdbsite=ZNS:Residues+That+Bind+The+Catalytic+Zn+Ion+(Zn+A+500)'>ZNS</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NVG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:54:03 2008'' |
Revision as of 07:54, 3 February 2008
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N249Y MUTANT OF THE ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS-TETRAGONAL CRYSTAL FORM
Overview
Alcohol dehydrogenase from Sulfolobus solfataricus (SsADH) is the only, enzyme from Archaea among the structurally studied members of the, medium-chain ADH family described so far. Here, we present the, three-dimensional structure of the apo form of the mutant N249Y which, exhibits increased catalytic activity when compared to the wild-type, enzyme. The substitution, located in the coenzyme binding domain, decreases the affinity for NAD(H) cofactor. The rearrangement of segments, 248-250 and 270-275, induced by the mutation, suggests an explanation for, the lower coenzyme affinity. This study also highlights the role in SsADH, catalysis of the flexible loops located at the interface between the, catalytic and the coenzyme domains.
About this Structure
1NVG is a Single protein structure of sequence from Sulfolobus solfataricus with as ligand. Active as Alcohol dehydrogenase, with EC number 1.1.1.1 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural study of a single-point mutant of Sulfolobus solfataricus alcohol dehydrogenase with enhanced activity., Esposito L, Bruno I, Sica F, Raia CA, Giordano A, Rossi M, Mazzarella L, Zagari A, FEBS Lett. 2003 Mar 27;539(1-3):14-8. PMID:12650918
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Categories: Alcohol dehydrogenase | Single protein | Sulfolobus solfataricus | Bruno, I. | Esposito, L. | Giordano, A. | Mazzarella, L. | Raia, C.A. | Rossi, M. | Sica, F. | Zagari, A. | ZN | Archaeon | Mutant | Nad(h) dependent | Zinc
