1o6i
From Proteopedia
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| - | [[Image:1o6i.jpg|left|200px]]<br /><applet load="1o6i" size=" | + | [[Image:1o6i.jpg|left|200px]]<br /><applet load="1o6i" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1o6i, resolution 1.70Å" /> | caption="1o6i, resolution 1.70Å" /> | ||
'''CHITINASE B FROM SERRATIA MARCESCENS COMPLEXED WITH THE CATALYTIC INTERMEDIATE MIMIC CYCLIC DIPEPTIDE CI4.'''<br /> | '''CHITINASE B FROM SERRATIA MARCESCENS COMPLEXED WITH THE CATALYTIC INTERMEDIATE MIMIC CYCLIC DIPEPTIDE CI4.'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1O6I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens] with SO4, DPR and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14. 3.2.1.14.] Known structural/functional Site: <scene name='pdbsite=ARB:ARG Binding Site For Chain A'>ARB</scene>. Full crystallographic information is available from [http:// | + | 1O6I is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=DPR:'>DPR</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.14. 3.2.1.14.] Known structural/functional Site: <scene name='pdbsite=ARB:ARG+Binding+Site+For+Chain+A'>ARB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O6I OCA]. |
==Reference== | ==Reference== | ||
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[[Category: inhibitor]] | [[Category: inhibitor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:54:08 2008'' |
Revision as of 07:54, 3 February 2008
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CHITINASE B FROM SERRATIA MARCESCENS COMPLEXED WITH THE CATALYTIC INTERMEDIATE MIMIC CYCLIC DIPEPTIDE CI4.
Overview
Family 18 chitinases are attractive targets for the development of new, inhibitors with chemotherapeutic potential against fungi, insects and, protozoan/nematodal parasites. Although several inhibitors have been, identified, these are based on complex chemistry, which hampers iterative, structure-based optimization. Here we report the details of chitinase, inhibition by the natural product peptide CI-4 [ cyclo -(L-Arg-D-Pro)], which possesses activity against the human pathogenic fungus Candida, albicans, and describe a 1.7 A (0.17 nm) crystal structure of CI-4 in, complex with the enzyme. The structure reveals that the cyclic dipeptide, inhibits chitinases by structurally mimicking a reaction intermediate, and, could, on the basis of its accessible chemistry, be a candidate for, further optimization.
About this Structure
1O6I is a Single protein structure of sequence from Serratia marcescens with , and as ligands. Active as Hydrolase, with EC number 3.2.1.14. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
The cyclic dipeptide CI-4 [cyclo-(l-Arg-d-Pro)] inhibits family 18 chitinases by structural mimicry of a reaction intermediate., Houston DR, Eggleston I, Synstad B, Eijsink VG, van Aalten DM, Biochem J. 2002 Nov 15;368(Pt 1):23-7. PMID:12323074
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