1o7k
From Proteopedia
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| - | [[Image:1o7k.gif|left|200px]]<br /><applet load="1o7k" size=" | + | [[Image:1o7k.gif|left|200px]]<br /><applet load="1o7k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1o7k, resolution 2.0Å" /> | caption="1o7k, resolution 2.0Å" /> | ||
'''HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES'''<br /> | '''HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1O7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=API:Second Anionic Lipid Binding Site. Chain C'>API</scene>. Full crystallographic information is available from [http:// | + | 1O7K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=API:Second+Anionic+Lipid+Binding+Site.+Chain+C'>API</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: sh3 domain]] | [[Category: sh3 domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:54:30 2008'' |
Revision as of 07:54, 3 February 2008
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HUMAN P47 PX DOMAIN COMPLEX WITH SULPHATES
Contents |
Overview
p47(phox) is a key cytosolic subunit required for activation of phagocyte, NADPH oxidase. The X-ray structure of the p47(phox) PX domain revealed two, distinct basic pockets on the membrane-binding surface, each occupied by a, sulfate. These two pockets have different specificities: one, preferentially binds phosphatidylinositol 3,4-bisphosphate, [PtdIns(3,4)P(2)] and is analogous to the phophatidylinositol 3-phosphate, (PtdIns3P)-binding pocket of p40(phox), while the other binds anionic, phospholipids such as phosphatidic acid (PtdOH) or phosphatidylserine. The, preference of this second site for PtdOH may be related to previously, observed activation of NADPH oxidase by PtdOH. Simultaneous occupancy of, the two phospholipid-binding pockets radically increases membrane, affinity. Strikingly, measurements for full-length p47(phox) show that, membrane interaction by the PX domain is masked by an intramolecular, association with the C-terminal SH3 domain (C-SH3). Either a site-specific, mutation in C-SH3 (W263R) or a mimic of the phosphorylated form of, p47(phox) [Ser(303, 304, 328, 359, 370)Glu] cause a transition from a, closed to an open conformation that binds membranes with a greater, affinity than the isolated PX domain.
Disease
Known disease associated with this structure: Chronic granulomatous disease due to deficiency of NCF-1 OMIM:[608512]
About this Structure
1O7K is a Single protein structure of sequence from Homo sapiens with as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Binding of the PX domain of p47(phox) to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction., Karathanassis D, Stahelin RV, Bravo J, Perisic O, Pacold CM, Cho W, Williams RL, EMBO J. 2002 Oct 1;21(19):5057-68. PMID:12356722
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